Rigidity of the nucleus during nuclear rotation in 3T3 cells

S. W. Paddock*, G. Albrecht-Buehler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Using near infrared microscopy and ultraviolet fluorescence microscopy of living 3T3 cells stained with the fluorochrome Hoechst 33342, we have demonstrated that the nucleoli and Hoechst 33342-stained chromocenters in the nucleus maintain a fixed pattern during nuclear rotation. We conclude that the term "nuclear rotation" refers to rotation of the entire nucleus in the cytoplasm of interphase cells, and that nuclear rotation is not an expression of karyoplasmic streaming. In conjunction with earlier results on nuclear rotation the data imply that the interface of nuclear rotation is located either between the two nuclear membranes or in the adjacent cytoplasm.

Original languageEnglish (US)
Pages (from-to)409-413
Number of pages5
JournalExperimental Cell Research
Volume175
Issue number2
DOIs
StatePublished - Apr 1988

ASJC Scopus subject areas

  • Cell Biology

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