RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong

Xi Chen, Qian Feng, Zhiyin Wu, Yingying Liu, Kun Huang, Rui Shi, Sidi Chen, Wen Lu, Mingxiao Ding, Richard A. Collins, Yin Wan Wendy Fung, Lok Ting Lau, Albert Cheung Hoi Yu, Jianguo Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A foot-and-mouth disease virus (FMDV, HKN/2002) was isolated in Hong Kong in 2002. The nucleotide sequence of the 3Dpol gene encoding the viral RNA-dependent RNA polymerase was determined and compared with that of the same gene from other FMDVs. The 3Dpol gene was 1410 nucleotides in length encoding a protein of 470 amino acid residues. Sequence comparisons indicated that HKN/2002 belonged to serotype O. An evolutionary tree based on the 3Dpol sequences of 20 FMDV isolates revealed that the nucleotide sequence of the HKN/2002 3Dpol gene was most similar to those of isolates found in Taiwan in 1997, suggesting that they share a common ancestor. The amino acid sequence of the HKN/2002 3Dpol gene was determined and aligned with those of representative isolates from seven other Picornaviridae genera. Eight highly conserved regions were detected, indicating a conserved functional relevance for these motifs. Alignment of 20 FMDV 3D pol amino acid sequences revealed a hypermutation region near the N-terminus that may help the virus evade host immune systems.

Original languageEnglish (US)
Pages (from-to)899-905
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume308
Issue number4
DOIs
StatePublished - Sep 5 2003

Keywords

  • 3D gene
  • Foot-and-mouth disease virus
  • Hypermutation
  • RNA-dependent RNA polymerase
  • Sequence alignment
  • Serotype

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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