RNA interference-mediated inhibition of hepatocyte nuclear factor 1α identifies target genes

Joanne R. Evans; David L. Kelly; Kirsten J. Morris; Elsa M. Arvide; Ann Harris

doi:10.1016/j.bbagrm.2008.03.002

RNA interference-mediated inhibition of hepatocyte nuclear factor 1α identifies target genes

Joanne R. Evans, David L. Kelly, Kirsten J. Morris, Elsa M. Arvide, Ann Harris^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Hepatocyte nuclear factor 1α (HNF1α) is a homeodomain transcription factor that is central to co-ordinated differentiation of a number of cell lineages, including hepatocytes in the liver and islet cells in the pancreas. HNF1α interacts directly with other transcription factors and co-factors and is involved in chromatin modification to alter gene expression. To further investigate the pivotal role of HNF1α in transcriptional control pathways we utilized RNA interference. An siRNA oligonucleotide specific for HNF1α reduced HNF1α protein levels by up to 70% in transient transfections of Caco2 cells. The same sequence incorporated into an shRNAi reduced protein levels by up to 90% in stable transfections. Microarray analysis of RNA from cell lines with stable RNAi-mediated down-regulation of HNF1α, identified genes known to be regulated by this transcription factor and also novel genes.

Original language	English (US)
Pages (from-to)	341-346
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume	1779
Issue number	5
DOIs	https://doi.org/10.1016/j.bbagrm.2008.03.002
State	Published - May 2008

Keywords

Hepatocyte nuclear factor α
RNA interference
Transcriptional network

ASJC Scopus subject areas

Genetics
Molecular Biology
Biophysics
Structural Biology
Biochemistry

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10.1016/j.bbagrm.2008.03.002

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title = "RNA interference-mediated inhibition of hepatocyte nuclear factor 1α identifies target genes",

abstract = "Hepatocyte nuclear factor 1α (HNF1α) is a homeodomain transcription factor that is central to co-ordinated differentiation of a number of cell lineages, including hepatocytes in the liver and islet cells in the pancreas. HNF1α interacts directly with other transcription factors and co-factors and is involved in chromatin modification to alter gene expression. To further investigate the pivotal role of HNF1α in transcriptional control pathways we utilized RNA interference. An siRNA oligonucleotide specific for HNF1α reduced HNF1α protein levels by up to 70% in transient transfections of Caco2 cells. The same sequence incorporated into an shRNAi reduced protein levels by up to 90% in stable transfections. Microarray analysis of RNA from cell lines with stable RNAi-mediated down-regulation of HNF1α, identified genes known to be regulated by this transcription factor and also novel genes.",

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AU - Evans, Joanne R.

AU - Kelly, David L.

AU - Morris, Kirsten J.

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AU - Harris, Ann

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AB - Hepatocyte nuclear factor 1α (HNF1α) is a homeodomain transcription factor that is central to co-ordinated differentiation of a number of cell lineages, including hepatocytes in the liver and islet cells in the pancreas. HNF1α interacts directly with other transcription factors and co-factors and is involved in chromatin modification to alter gene expression. To further investigate the pivotal role of HNF1α in transcriptional control pathways we utilized RNA interference. An siRNA oligonucleotide specific for HNF1α reduced HNF1α protein levels by up to 70% in transient transfections of Caco2 cells. The same sequence incorporated into an shRNAi reduced protein levels by up to 90% in stable transfections. Microarray analysis of RNA from cell lines with stable RNAi-mediated down-regulation of HNF1α, identified genes known to be regulated by this transcription factor and also novel genes.

KW - Hepatocyte nuclear factor α

KW - RNA interference

KW - Transcriptional network

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RNA interference-mediated inhibition of hepatocyte nuclear factor 1α identifies target genes

Abstract

Keywords

ASJC Scopus subject areas

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