RNA nanoparticle as a vector for targeted siRNA delivery into glioblastoma mouse model

Tae Jin Lee, Farzin Haque, Dan Shu, Ji Young Yoo, Hui Li, Robert A. Yokel, Craig Horbinski, Tae Hyong Kim, Sung Hak Kim, Chang Hyuk Kwon, Ichiro Nakano, Balveen Kaur, Peixuan Guo, Carlo M. Croce*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Systemic siRNA administration to target and treat glioblastoma, one of the most deadly cancers, requires robust and efficient delivery platform without immunogenicity. Here we report newly emerged multivalent naked RNA nanoparticle (RNP) based on pRNA 3-way-junction (3WJ) from bacteriophage phi29 to target glioblastoma cells with folate (FA) ligand and deliver siRNA for gene silencing. Systemically injected FA-pRNA-3WJ RNPs successfully targeted and delivered siRNA into brain tumor cells in mice, and efficiently reduced luciferase reporter gene expression (4-fold lower than control). The FA-pRNA-3WJ RNP also can target human patient-derived glioblastoma stem cells, thought to be responsible for tumor initiation and deadly recurrence, without accumulation in adjacent normal brain cells, nor other major internal organs. This study provides possible application of pRNA-3WJ RNP for specific delivery of therapeutics such as siRNA, microRNA and/or chemotherapeutic drugs into glioblastoma cells without inflicting collateral damage to healthy tissues.

Original languageEnglish (US)
Pages (from-to)14766-14776
Number of pages11
JournalOncotarget
Volume6
Issue number17
DOIs
StatePublished - 2015

Keywords

  • Glioblastoma
  • Nanoparticle
  • PRNA
  • SiRNA
  • Three-way junction

ASJC Scopus subject areas

  • Oncology

Fingerprint

Dive into the research topics of 'RNA nanoparticle as a vector for targeted siRNA delivery into glioblastoma mouse model'. Together they form a unique fingerprint.

Cite this