RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E

Jason R. Kennerdell, Shinji Yamaguchi, Richard W. Carthew*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

150 Scopus citations

Abstract

Gene silencing by double-stranded RNA is a widespread phenomenon called RNAi, involving homology-dependent degradation of mRNAs. Here we show that RNAi is established in the Drosophila female germ line. mRNA transcripts are translationally quiescent at the arrested oocyte stage and are insensitive to RNAi. Upon oocyte maturation, transcripts that are translated become sensitive to degradation while untranslated transcripts remain resistant. Mutations in aubergine and spindle-E, members of the PIWI/PAZ and DE-H helicase gene families, respectively, block RNAi activation during egg maturation and perturb translation control during oogenesis, supporting a connection between gene silencing and translation in the oocyte.

Original languageEnglish (US)
Pages (from-to)1884-1889
Number of pages6
JournalGenes and Development
Volume16
Issue number15
DOIs
StatePublished - Aug 1 2002

Keywords

  • Aubergine
  • Drosophila
  • Oogenesis
  • RNAi
  • Spindle-E

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E'. Together they form a unique fingerprint.

Cite this