Role of 4.5S RNA in assembly of the bacterial signal recognition particle with its receptor

Paul Peluso; Daniel Herschlag; Silke Nock; Douglas M. Freymann; Arthur E. Johnson; Peter Walter

doi:10.1126/science.288.5471.1640

Role of 4.5S RNA in assembly of the bacterial signal recognition particle with its receptor

Paul Peluso, Daniel Herschlag, Silke Nock, Douglas M. Freymann, Arthur E. Johnson, Peter Walter^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

127 Scopus citations

Abstract

The mechanism by which a signal recognition particle (SRP) and its receptor mediate protein targeting to the endoplasmic reticulum or to the bacterial plasma membrane is evolutionarily conserved. In Escherichia coli, this reaction is mediated by the Ffh/4.5S RNA ribonucleoprotein complex (Ffh/4.5S RNP; the SRP) and the FtsY protein (the SRP receptor). We have quantified the effects of 4.5S RNA on Ffh-FtsY complex formation by monitoring changes in tryptophan fluorescence. Surprisingly, 4.5S RNA facilitates both assembly and disassembly of the Ffh-FtsY complex to a similar extent. These results provide an example of an RNA molecule facilitating protein-protein interactions in a catalytic fashion.

Original language	English (US)
Pages (from-to)	1640-1643
Number of pages	4
Journal	Science
Volume	288
Issue number	5471
DOIs	https://doi.org/10.1126/science.288.5471.1640
State	Published - Jun 2 2000
Externally published	Yes

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title = "Role of 4.5S RNA in assembly of the bacterial signal recognition particle with its receptor",

abstract = "The mechanism by which a signal recognition particle (SRP) and its receptor mediate protein targeting to the endoplasmic reticulum or to the bacterial plasma membrane is evolutionarily conserved. In Escherichia coli, this reaction is mediated by the Ffh/4.5S RNA ribonucleoprotein complex (Ffh/4.5S RNP; the SRP) and the FtsY protein (the SRP receptor). We have quantified the effects of 4.5S RNA on Ffh-FtsY complex formation by monitoring changes in tryptophan fluorescence. Surprisingly, 4.5S RNA facilitates both assembly and disassembly of the Ffh-FtsY complex to a similar extent. These results provide an example of an RNA molecule facilitating protein-protein interactions in a catalytic fashion.",

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AU - Peluso, Paul

AU - Herschlag, Daniel

AU - Nock, Silke

AU - Freymann, Douglas M.

AU - Johnson, Arthur E.

AU - Walter, Peter

PY - 2000/6/2

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AB - The mechanism by which a signal recognition particle (SRP) and its receptor mediate protein targeting to the endoplasmic reticulum or to the bacterial plasma membrane is evolutionarily conserved. In Escherichia coli, this reaction is mediated by the Ffh/4.5S RNA ribonucleoprotein complex (Ffh/4.5S RNP; the SRP) and the FtsY protein (the SRP receptor). We have quantified the effects of 4.5S RNA on Ffh-FtsY complex formation by monitoring changes in tryptophan fluorescence. Surprisingly, 4.5S RNA facilitates both assembly and disassembly of the Ffh-FtsY complex to a similar extent. These results provide an example of an RNA molecule facilitating protein-protein interactions in a catalytic fashion.

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Role of 4.5S RNA in assembly of the bacterial signal recognition particle with its receptor

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