Role of antibody in cytotoxicity by lymphocytes armed against 253j bladder cancer line

W. J. Catalona; T. L. Ratliff; R. E. McCool; W. D W Heston

doi:10.1159/000232829

Role of antibody in cytotoxicity by lymphocytes armed against 253j bladder cancer line

W. J. Catalona^*, T. L. Ratliff, R. E. McCool, W. D W Heston

^*Corresponding author for this work

Urology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The role of antibody-dependent cell-mediated cytotoxicity (ADCC) in cytotoxicity mediated by antibody-armed effector cells was examined. Cytotoxicity mediated by unsensitized human peripheral blood mononuclear cells (MNC) directed against the human bladder cancer cell line, 253J, was significantly enhanced when MNC were pre-incubated with a rabbit anti-253J serum and then washed free of unbound antibody. Non-adherent, Fc receptor positive MNC were the effector cells and the arming antibody was IgG. Supernatants of 24-hour cultures and armed MNC exhibited antibody activity in ADCC assays, suggesting the ADCC was in part responsible for the arming phenomenon.

Original language	English (US)
Pages (from-to)	259-266
Number of pages	8
Journal	International archives of allergy and immunology
Volume	66
Issue number	3
DOIs	https://doi.org/10.1159/000232829
State	Published - 1981

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1159/000232829

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title = "Role of antibody in cytotoxicity by lymphocytes armed against 253j bladder cancer line",

abstract = "The role of antibody-dependent cell-mediated cytotoxicity (ADCC) in cytotoxicity mediated by antibody-armed effector cells was examined. Cytotoxicity mediated by unsensitized human peripheral blood mononuclear cells (MNC) directed against the human bladder cancer cell line, 253J, was significantly enhanced when MNC were pre-incubated with a rabbit anti-253J serum and then washed free of unbound antibody. Non-adherent, Fc receptor positive MNC were the effector cells and the arming antibody was IgG. Supernatants of 24-hour cultures and armed MNC exhibited antibody activity in ADCC assays, suggesting the ADCC was in part responsible for the arming phenomenon.",

author = "Catalona, {W. J.} and Ratliff, {T. L.} and McCool, {R. E.} and Heston, {W. D W}",

year = "1981",

doi = "10.1159/000232829",

language = "English (US)",

volume = "66",

pages = "259--266",

journal = "International archives of allergy and immunology",

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T1 - Role of antibody in cytotoxicity by lymphocytes armed against 253j bladder cancer line

AU - Catalona, W. J.

AU - Ratliff, T. L.

AU - McCool, R. E.

AU - Heston, W. D W

PY - 1981

Y1 - 1981

N2 - The role of antibody-dependent cell-mediated cytotoxicity (ADCC) in cytotoxicity mediated by antibody-armed effector cells was examined. Cytotoxicity mediated by unsensitized human peripheral blood mononuclear cells (MNC) directed against the human bladder cancer cell line, 253J, was significantly enhanced when MNC were pre-incubated with a rabbit anti-253J serum and then washed free of unbound antibody. Non-adherent, Fc receptor positive MNC were the effector cells and the arming antibody was IgG. Supernatants of 24-hour cultures and armed MNC exhibited antibody activity in ADCC assays, suggesting the ADCC was in part responsible for the arming phenomenon.

AB - The role of antibody-dependent cell-mediated cytotoxicity (ADCC) in cytotoxicity mediated by antibody-armed effector cells was examined. Cytotoxicity mediated by unsensitized human peripheral blood mononuclear cells (MNC) directed against the human bladder cancer cell line, 253J, was significantly enhanced when MNC were pre-incubated with a rabbit anti-253J serum and then washed free of unbound antibody. Non-adherent, Fc receptor positive MNC were the effector cells and the arming antibody was IgG. Supernatants of 24-hour cultures and armed MNC exhibited antibody activity in ADCC assays, suggesting the ADCC was in part responsible for the arming phenomenon.

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JO - International archives of allergy and immunology

JF - International archives of allergy and immunology

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ER -

Role of antibody in cytotoxicity by lymphocytes armed against 253j bladder cancer line

Abstract

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