Role of antibody in cytotoxicity by lymphocytes armed against 253j bladder cancer line

W. J. Catalona*, T. L. Ratliff, R. E. McCool, W. D W Heston

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The role of antibody-dependent cell-mediated cytotoxicity (ADCC) in cytotoxicity mediated by antibody-armed effector cells was examined. Cytotoxicity mediated by unsensitized human peripheral blood mononuclear cells (MNC) directed against the human bladder cancer cell line, 253J, was significantly enhanced when MNC were pre-incubated with a rabbit anti-253J serum and then washed free of unbound antibody. Non-adherent, Fc receptor positive MNC were the effector cells and the arming antibody was IgG. Supernatants of 24-hour cultures and armed MNC exhibited antibody activity in ADCC assays, suggesting the ADCC was in part responsible for the arming phenomenon.

Original languageEnglish (US)
Pages (from-to)259-266
Number of pages8
JournalInternational archives of allergy and immunology
Volume66
Issue number3
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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