Role of cortical tension in fibroblast shape and movement.

G. Albrecht-Buehler*

*Corresponding author for this work

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

In order to analyze the cellular mechanisms of shape formation, the shape of individual 3T3 cells was perturbed by micromanipulation resulting in the detachment and relaxation of a cellular extension and the bending of the extension to form an "elbow" at a variable angle beta. Finally, the tip of the extension was allowed to reattach to the substrate away from the cell. The cells reacted by drawing the extension tight. If beta less than 90 degrees, the elbow moved laterally for 8-15 min until the extension projected orthogonally at the cell surface. If beta greater than or equal to 90 degrees, the extension remained stationary. Finally, in all cases webs formed between attachment points in the perturbed area. If the tip of the extension was allowed to touch its own cell body, thus forming a loop, the cells invariably closed the loop. The paper interprets the cellular reaction as the result of cortical tension and suggests that it is a major factor in the formation of fibroblast shape and the expressions of fibroblast motility.

Original languageEnglish (US)
Pages (from-to)54-67
Number of pages14
JournalCell Motility and the Cytoskeleton
Volume7
Issue number1
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

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