Role of dopant concentration, crystal phase and particle size on microbial inactivation of Cu-doped TiO2 nanoparticles

Manoranjan Sahu*, Bing Wu, Liying Zhu, Craig Jacobson, Wei Ning Wang, Kristen Jones, Yogesh Goyal, Yinjie J. Tang, Pratim Biswas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The properties of Cu-doped TiO2 nanoparticles (NPs) were independently controlled in a flame aerosol reactor by varying the molar feed ratios of the precursors, and by optimizing temperature and time history in the flame. The effect of the physico-chemical properties (dopant concentration, crystal phase and particle size) of Cu-doped TiO2 nanoparticles on inactivation of Mycobacterium smegmatis (a model pathogenic bacterium) was investigated under three light conditions (complete dark, fluorescent light and UV light). The survival rate of M. smegmatis (in a minimal salt medium for 2h) exposed to the NPs varied depending on the light irradiation conditions as well as the dopant concentrations. In dark conditions, pristine TiO2 showed insignificant microbial inactivation, but inactivation increased with increasing dopant concentration. Under fluorescent light illumination, no significant effect was observed for TiO2. However, when TiO 2 was doped with copper, inactivation increased with dopant concentration, reaching more than 90% (>3wt% dopant). Enhanced microbial inactivation by TiO2 NPs was observed only under UV light. When TiO2 NPs were doped with copper, their inactivation potential was promoted and the UV-resistant cells were reduced by over99%. In addition, the microbial inactivation potential of NPs was also crystal-phase-and size-dependent under all three light conditions. A lower ratio of anatase phase and smaller sizes of Cu-doped TiO2 NPs resulted in decreased bacterial survival. The increased inactivation potential of doped TiO 2 NPs is possibly due to both enhanced photocatalytic reactions and leached copper ions.

Original languageEnglish (US)
Article number415704
Issue number41
StatePublished - Oct 14 2011
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • General Chemistry
  • General Materials Science
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering


Dive into the research topics of 'Role of dopant concentration, crystal phase and particle size on microbial inactivation of Cu-doped TiO2 nanoparticles'. Together they form a unique fingerprint.

Cite this