Role of fatty acid structure in the reversible activation of phosphatidylcholine synthesis in lymphocytes

Kristin E. Anderson, Donna S. Whitlon, Gerald C. Mueller*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fatty acids rapidly accelerate (1.5-7.0-fold) the incorporation of [methyl-3H]choline chloride into the phosphatidylcholine fraction of bovine lymphocyte lipids. This ability of fatty acids to activate choline phospholipid synthesis has been correlated with certain structural features of fatty acids. Mono- and polyenoic unsaturated fatty acids of 18 and 20 carbons in length are highly active, whereas their saturated analogues are nearly inactive. Among the unsaturated fatty acids, the cis-isomers are active, while the trans-isomers are relatively ineffective. The delayed addition of bovine serum albumin (5 mg/ml) and other lipid-binding proteins to activated cells rapidly counteracts the lipid effects. The activated state of the cell membrane thus appears to be a dynamic one, requiring the continued interaction of the fatty acid with a lipid-sensitive target molecule of the cell surface that in turn appears to coordinate the enzymatic components of this pathway.

Original languageEnglish (US)
Pages (from-to)360-368
Number of pages9
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume835
Issue number2
DOIs
StatePublished - Jul 9 1985

Fingerprint

Lymphocytes
Phosphatidylcholines
Fatty Acids
Chemical activation
Lipids
Choline
Unsaturated Fatty Acids
Isomers
Cell membranes
Bovine Serum Albumin
Phospholipids
Carrier Proteins
Carbon
Cell Membrane
Molecules

Keywords

  • (Bovine lymphocyte)
  • Fatty acid structure
  • Phosphatidylcholine
  • Phospholipid synthesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

Cite this

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title = "Role of fatty acid structure in the reversible activation of phosphatidylcholine synthesis in lymphocytes",
abstract = "Fatty acids rapidly accelerate (1.5-7.0-fold) the incorporation of [methyl-3H]choline chloride into the phosphatidylcholine fraction of bovine lymphocyte lipids. This ability of fatty acids to activate choline phospholipid synthesis has been correlated with certain structural features of fatty acids. Mono- and polyenoic unsaturated fatty acids of 18 and 20 carbons in length are highly active, whereas their saturated analogues are nearly inactive. Among the unsaturated fatty acids, the cis-isomers are active, while the trans-isomers are relatively ineffective. The delayed addition of bovine serum albumin (5 mg/ml) and other lipid-binding proteins to activated cells rapidly counteracts the lipid effects. The activated state of the cell membrane thus appears to be a dynamic one, requiring the continued interaction of the fatty acid with a lipid-sensitive target molecule of the cell surface that in turn appears to coordinate the enzymatic components of this pathway.",
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Role of fatty acid structure in the reversible activation of phosphatidylcholine synthesis in lymphocytes. / Anderson, Kristin E.; Whitlon, Donna S.; Mueller, Gerald C.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 835, No. 2, 09.07.1985, p. 360-368.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Role of fatty acid structure in the reversible activation of phosphatidylcholine synthesis in lymphocytes

AU - Anderson, Kristin E.

AU - Whitlon, Donna S.

AU - Mueller, Gerald C.

PY - 1985/7/9

Y1 - 1985/7/9

N2 - Fatty acids rapidly accelerate (1.5-7.0-fold) the incorporation of [methyl-3H]choline chloride into the phosphatidylcholine fraction of bovine lymphocyte lipids. This ability of fatty acids to activate choline phospholipid synthesis has been correlated with certain structural features of fatty acids. Mono- and polyenoic unsaturated fatty acids of 18 and 20 carbons in length are highly active, whereas their saturated analogues are nearly inactive. Among the unsaturated fatty acids, the cis-isomers are active, while the trans-isomers are relatively ineffective. The delayed addition of bovine serum albumin (5 mg/ml) and other lipid-binding proteins to activated cells rapidly counteracts the lipid effects. The activated state of the cell membrane thus appears to be a dynamic one, requiring the continued interaction of the fatty acid with a lipid-sensitive target molecule of the cell surface that in turn appears to coordinate the enzymatic components of this pathway.

AB - Fatty acids rapidly accelerate (1.5-7.0-fold) the incorporation of [methyl-3H]choline chloride into the phosphatidylcholine fraction of bovine lymphocyte lipids. This ability of fatty acids to activate choline phospholipid synthesis has been correlated with certain structural features of fatty acids. Mono- and polyenoic unsaturated fatty acids of 18 and 20 carbons in length are highly active, whereas their saturated analogues are nearly inactive. Among the unsaturated fatty acids, the cis-isomers are active, while the trans-isomers are relatively ineffective. The delayed addition of bovine serum albumin (5 mg/ml) and other lipid-binding proteins to activated cells rapidly counteracts the lipid effects. The activated state of the cell membrane thus appears to be a dynamic one, requiring the continued interaction of the fatty acid with a lipid-sensitive target molecule of the cell surface that in turn appears to coordinate the enzymatic components of this pathway.

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KW - Phosphatidylcholine

KW - Phospholipid synthesis

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