Role of gut flora on intestinal group II phospholipase A2 activity and intestinal injury in shock

Ranna A. Rozenfeld, Xueli Liu, Isabelle De Plaen, Wei Hsueh*

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We previously showed that group II phospholipase A2 (PLA2-II), a secretory, bactericidal, and proinflammatory protein in intestinal crypts, is upregulated after lipopolysaccharide (LPS) and platelet-activating factor (PAF) challenge. Here we examined whether germ-free environment (GF) or antibiotic treatment (ABX) affects the pathophysiological responses and intestinal PLA2-II activity after PAF (1.5 μg/kg) or LPS (8 mg/kg) injection. We found that LPS and PAF induced hypotension and mild intestinal injury in conventionally fed (CN) rats; these changes were milder in ABX rats, whereas GF rats showed no intestinal injury. PLA2-II enzyme activity was detected in normal rat small intestine; the basal level was not diminished in ABX or GF rats. PAF and LPS caused an increase in PLA2-II activity, which was abrogated in GF and ABX rats. Recolonization of GF rats by enteral contamination restituted their PLA2-II response to PAF and LPS and susceptibility to bowel injury. We conclude that PAF- and LPS-induced increases in PLA2-II activity are dependent on gut bacteria, and ABX and GF rats are less susceptible to LPS-induced injury than CN rats.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume281
Issue number4 44-4
StatePublished - Oct 22 2001

Keywords

  • Bacteria
  • Germ-free environment
  • Lipopolysaccharide
  • Mixed-antibiotic treatment
  • Platelet-activating factor

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology
  • Physiology (medical)

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