Role of ICAM-1 and P-selectin expression in the development and effector function of CD4+CD25+regulatory T cells

Adam P. Kohm, Stephen D. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Dynamic regulatory mechanisms prevent autoreactive T cell activation. Upon T cell receptor crosslinking, CD4+CD25+T regulatory (TR) cells block both the proliferation and cytokine production of CD4+CD25-effector cells in an apparent antigen non-specific manner. Within the TRpopulation, L-selectin (CD62L) hiTRcells have been described as more efficient suppressors of T cell proliferation than CD62LlowTRcells. We have previously reported that CD4+CD25+CD62L hiTRcells express elevated levels of two additional adhesion molecules, ICAM-1 (CD54) and P-selectin (CD62P) in comparison to non-TRcells. In the current study, we investigated the functional contribution of CD54 and CD62P expression to the suppressive phenotype of T Rcells both in vitro and in vivo. While the CD4+CD25 +TRcell population was demonstrated to be significantly larger in CD62P-/-mice than in wild-type C57BL/6 mice, CD62P -/-TRcell function was deficient in vitro, but not in vivo. Interestingly, we detected no deficiencies in TRcell numbers or effector function in CD54-/-mice suggesting that T Rcells may differ from effector CD4+T cells in the requirement for CD54 expression within the immunological synapse. Collectively, these findings indicate that CD62P may influence TRcell differentiation/development and that TRcell activation occurs independently of CD54 expression.

Original languageEnglish (US)
Pages (from-to)261-271
Number of pages11
JournalJournal of Autoimmunity
Volume21
Issue number3
DOIs
StatePublished - Nov 2003

Keywords

  • Adhesion molecules
  • Autoimmune disease
  • CD54
  • CD62P
  • Regulatory T cell
  • T-lymphocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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