Role of importin-β in coupling ran to downstream targets in microtubule assembly

C. Wiese, A. Wilde, M. S. Moore, S. A. Adam, A. Merdes*, Y. Zheng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

264 Scopus citations

Abstract

The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclear-mitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-β. We show that importin-β is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-β and NuMA. Importin-β therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis.

Original languageEnglish (US)
Pages (from-to)653-656
Number of pages4
JournalScience
Volume291
Issue number5504
DOIs
StatePublished - Jan 26 2001

ASJC Scopus subject areas

  • General

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