Role of kinesin light chain-2 of kinesin-1 in the traffic of Na,K-ATPase-containing vesicles in alveolar epithelial cells

Humberto E. Trejo, Emilia Lecuona, Doris Grillo, Igal Szleifer, Oksana E. Nekrasova, Vladimir I. Gelfand, Jacob I. Sznajder

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Recruitment of the Na,K-ATPase to the plasma membrane of alveolar epithelial cells results in increased active Na+ transport and fluid clearance in a process that requires an intact microtubule network. However, the microtubule motors involved in this process have not been identified. In the present report, we studied the role of kinesin-1, a plus-end microtubule molecular motor that has been implicated in the movement of organelles in the Na,K-ATPase traffic. We determined by confocal microscopy and biochemical assays that kinesin-1 and the Na,K-ATPase are present in the same membranous cellular compartment. Knock-down of kinesin-1 heavy chain (KHC) or the light chain-2 (KLC2), but not of the light chain-1 (KLC1), decreased the movement of Na,K-ATPase-containing vesicles when compared to sham siRNA-transfected cells (control group). Thus, a specific isoform of kinesin-1 is required for microtubule-dependent recruitment of Na,K-ATPase to the plasma membrane, which is of physiological significance.

Original languageEnglish (US)
Pages (from-to)374-382
Number of pages9
JournalFASEB Journal
Volume24
Issue number2
DOIs
StatePublished - Feb 2010

Keywords

  • Intracellular traffic
  • Molecular motors
  • Sodium pump

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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