Role of protein kinase activation in the induction of B cell adhesion by MHC class II ligands

Ramsay Fuleihan, François Spertini, Raif S. Geha, Talal Chatila

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Engagement of MHC class II (Ia) molecules on B cells induces tyrosine phosphorylation, phosphoinositide turnover, elevation of intracellular calcium concentrations, and a rise in cAMP levels. However, a role for these biochemical signals in mediating functional responses induced by Ia ligands remains largely undefined. In this study, we utilized the induction of B cell adhesion by Ia ligands to demonstrate a role for signals transduced via Ia molecules in the generation of a functional response. Ia ligands that induced B cell aggregation induced tyrosine phosphorylation, whereas Ia ligands that did not induce B cell aggregation failed to induce any detectable tyrosine phosphorylation. Ia-induced B cell aggregation and tyrosine phosphorylation were inhibited by genistein and by herbimycin A, inhibitors of tyrosine kinases (PTK). Sphingosine and calphostin C, inhibitors of protein kinase C (PKC), also inhibited Ia-induced adhesion whereas HA1004, an inhibitor of cyclic nucleotide-dependent kinases, did not. Ia ligands induced both LFA-1- dependent and LFA-1-independent B cell adhesion. These two pathways of cell adhesion differed in their requirement for activation signals. PKC activation was sufficient for LFA-1-dependent adhesion, whereas LFA-1-independent adhesion required independent phosphorylation events mediated by PKC and by PTK. These results provide functional relevance for biochemical signals transduced via Ia molecules by demonstrating that Ia-induced B cell adhesion is mediated by the activation of PKC and by one or more PTK.

Original languageEnglish (US)
Pages (from-to)1853-1858
Number of pages6
JournalJournal of Immunology
Volume149
Issue number6
StatePublished - Jan 1 1992

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Cell Adhesion
Protein Kinases
B-Lymphocytes
Lymphocyte Function-Associated Antigen-1
Ligands
Cell Aggregation
Protein Kinase C
Phosphorylation
Tyrosine
Sphingosine
Genistein
Cyclic Nucleotides
Phosphatidylinositols
Protein-Tyrosine Kinases
Phosphotransferases
Calcium

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Fuleihan, Ramsay ; Spertini, François ; Geha, Raif S. ; Chatila, Talal. / Role of protein kinase activation in the induction of B cell adhesion by MHC class II ligands. In: Journal of Immunology. 1992 ; Vol. 149, No. 6. pp. 1853-1858.
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Role of protein kinase activation in the induction of B cell adhesion by MHC class II ligands. / Fuleihan, Ramsay; Spertini, François; Geha, Raif S.; Chatila, Talal.

In: Journal of Immunology, Vol. 149, No. 6, 01.01.1992, p. 1853-1858.

Research output: Contribution to journalArticle

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