Role of sustained antigen release from nanoparticle vaccines in shaping the T cell memory phenotype

Stacey L. Demento, Weiguo Cui, Jason M. Criscione, Eric Stern, Jacob Tulipan, Susan M. Kaech, Tarek M. Fahmy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

251 Scopus citations

Abstract

Particulate vaccines are emerging promising technologies for the creation of tunable prophylactics against a wide variety of conditions. Vesicular and solid biodegradable polymer platforms, exemplified by liposomes and polyesters, respectively, are two of the most ubiquitous platforms in vaccine delivery studies. Here we directly compared the efficacy of each in a long-term immunization study and in protection against a model bacterial antigen. Immunization with poly(lactide-co-glycolide) (PLGA) nanoparticles elicited prolonged antibody titers compared to liposomes and alum. The magnitude of the cellular immune response was also highest in mice vaccinated with PLGA, which also showed a higher frequency of effector-like memory T cell phenotype, leading to an effective clearance of intracellular bacteria. The difference in performance of these two common particulate platforms is shown not to be due to material differences but appears to be connected to the kinetics of antigen delivery. Thus, this study highlights the importance of sustained antigen release mediated by particulate platforms and its role in the long-term appearance of effector memory cellular response.

Original languageEnglish (US)
Pages (from-to)4957-4964
Number of pages8
JournalBiomaterials
Volume33
Issue number19
DOIs
StatePublished - Jun 2012

Keywords

  • Liposome
  • Memory T cells
  • Nanoparticle
  • PLGA
  • Persistence
  • Vaccine

ASJC Scopus subject areas

  • Mechanics of Materials
  • Ceramics and Composites
  • Bioengineering
  • Biophysics
  • Biomaterials

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