Role of the α(v)β3 integrin in human melanoma cell invasion

R. E.B. Seftor*, E. A. Seftor, K. R. Gehlsen, W. G. Stetler-Stevenson, P. D. Brown, E. Ruoslahti, M. J.C. Hendrix

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

425 Scopus citations

Abstract

The human melanoma cell line A375M expresses the vitronectin receptor (α(v)β3 integrin) on its cell surface. Treatment of A375M cells with either polyclonal or monoclonal anti-α(v)β3 antibodies resulted in stimulation of invasion through basement membrane matrices in vitro. Similar treatment of these cells with a monoclonal anti-α(v) antibody, which does not inhibit the adhesive function of the α(v)β3 antigen, also stimulated invasion; however, anti-β3 antibody treatment had no effect. Furthermore, pretreatment of the cells with vitronectin or addition of vitronectin to the basement membrane matrix also resulted in stimulation of invasion. Similar treatments with fibronectin receptor antibody or fibronectin had no effect on invasion. Analysis of type IV collagenase expression in cells treated with anti-α(v)β3 antibody showed higher levels of both the secreted 72-kDa enzyme and its mRNA. Signal transduction through the α(v)β3 integrin could underlie the elevated expression of metalloproteinase and the enhanced invasion of A375M cells through basement membrane matrices.

Original languageEnglish (US)
Pages (from-to)1557-1561
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number5
DOIs
StatePublished - 1992

Keywords

  • collagenase IV
  • signal transduction
  • vitronectin
  • vitronectin receptor

ASJC Scopus subject areas

  • General

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