Role of the Tertiary Nucleotides in the Interaction of Yeast Phenylalanine tRNA with Its Cognate Synthetase

Jeffrey R. Sampson, Anthony B. DiRenzo, Linda S. Behlen, Olke C. Uhlenbeck

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

In vitro transcription by T7 RNA polymerase was used to prepare 32 different mutations in the 21 nucleotides that participate in the 9 tertiary base pairs or triples of yeast tRNAphe. The mutations were designed either to disrupt the tertiary interaction or to change the sequence without disrupting the structure by transplanting tertiary interactions present in other tRNAs. Steady-state aminoacylation kinetics with purified yeast phenylalanyl synthetase revealed little change in reaction rate as long as a tertiary interaction was maintained. This suggests that the tertiary nucleotides only contribute to the folding of tRNAPhe and do not participate directly in sequence-specific interaction with the synthetase.

Original languageEnglish (US)
Pages (from-to)2523-2532
Number of pages10
JournalBiochemistry
Volume29
Issue number10
DOIs
StatePublished - Mar 13 1990

ASJC Scopus subject areas

  • Biochemistry

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