Role of x-ray-induced damage in biological micro-imaging

Richard A. London; James E. Trebes; Chris J. Jacobsen

Role of x-ray-induced damage in biological micro-imaging

Richard A. London^*, James E. Trebes, Chris J. Jacobsen

^*Corresponding author for this work

Physics and Astronomy

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

10 Scopus citations

Abstract

Interactions of x rays with a sample are studied to determine the optimal wavelength, source energy, and exposure time for microscopy and holography. The optimal wavelength is influenced by two criteria: minimizing the required source energy and minimizing the absorbed dose and subsequent damage to the sample. The use of heavy element labels, such as colloidal gold, can significantly reduce the energy and dose. Limits to the exposure time due to natural motions, x-ray induced chemical damage, heat build-up, and hydrodynamic expansion are discussed.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Publisher	Publ by Int Soc for Optical Engineering
Pages	333-340
Number of pages	8
Volume	1741
ISBN (Print)	0819409146
State	Published - Jan 1 1993
Event	Soft X-Ray Microscopy - San Diego, CA, USA Duration: Jul 19 1992 → Jul 21 1992

Other

Other	Soft X-Ray Microscopy
City	San Diego, CA, USA
Period	7/19/92 → 7/21/92

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

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Cite this

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title = "Role of x-ray-induced damage in biological micro-imaging",

abstract = "Interactions of x rays with a sample are studied to determine the optimal wavelength, source energy, and exposure time for microscopy and holography. The optimal wavelength is influenced by two criteria: minimizing the required source energy and minimizing the absorbed dose and subsequent damage to the sample. The use of heavy element labels, such as colloidal gold, can significantly reduce the energy and dose. Limits to the exposure time due to natural motions, x-ray induced chemical damage, heat build-up, and hydrodynamic expansion are discussed.",

author = "London, {Richard A.} and Trebes, {James E.} and Jacobsen, {Chris J.}",

year = "1993",

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language = "English (US)",

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volume = "1741",

pages = "333--340",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "Publ by Int Soc for Optical Engineering",

note = "Soft X-Ray Microscopy ; Conference date: 19-07-1992 Through 21-07-1992",

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T1 - Role of x-ray-induced damage in biological micro-imaging

AU - London, Richard A.

AU - Trebes, James E.

AU - Jacobsen, Chris J.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Interactions of x rays with a sample are studied to determine the optimal wavelength, source energy, and exposure time for microscopy and holography. The optimal wavelength is influenced by two criteria: minimizing the required source energy and minimizing the absorbed dose and subsequent damage to the sample. The use of heavy element labels, such as colloidal gold, can significantly reduce the energy and dose. Limits to the exposure time due to natural motions, x-ray induced chemical damage, heat build-up, and hydrodynamic expansion are discussed.

AB - Interactions of x rays with a sample are studied to determine the optimal wavelength, source energy, and exposure time for microscopy and holography. The optimal wavelength is influenced by two criteria: minimizing the required source energy and minimizing the absorbed dose and subsequent damage to the sample. The use of heavy element labels, such as colloidal gold, can significantly reduce the energy and dose. Limits to the exposure time due to natural motions, x-ray induced chemical damage, heat build-up, and hydrodynamic expansion are discussed.

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M3 - Conference contribution

AN - SCOPUS:0027202882

SN - 0819409146

VL - 1741

SP - 333

EP - 340

BT - Proceedings of SPIE - The International Society for Optical Engineering

PB - Publ by Int Soc for Optical Engineering

T2 - Soft X-Ray Microscopy

Y2 - 19 July 1992 through 21 July 1992

ER -