TY - GEN
T1 - Role of x-ray-induced damage in biological micro-imaging
AU - London, Richard A.
AU - Trebes, James E.
AU - Jacobsen, Chris J.
PY - 1993
Y1 - 1993
N2 - Interactions of x rays with a sample are studied to determine the optimal wavelength, source energy, and exposure time for microscopy and holography. The optimal wavelength is influenced by two criteria: minimizing the required source energy and minimizing the absorbed dose and subsequent damage to the sample. The use of heavy element labels, such as colloidal gold, can significantly reduce the energy and dose. Limits to the exposure time due to natural motions, x-ray induced chemical damage, heat build-up, and hydrodynamic expansion are discussed.
AB - Interactions of x rays with a sample are studied to determine the optimal wavelength, source energy, and exposure time for microscopy and holography. The optimal wavelength is influenced by two criteria: minimizing the required source energy and minimizing the absorbed dose and subsequent damage to the sample. The use of heavy element labels, such as colloidal gold, can significantly reduce the energy and dose. Limits to the exposure time due to natural motions, x-ray induced chemical damage, heat build-up, and hydrodynamic expansion are discussed.
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M3 - Conference contribution
AN - SCOPUS:0027202882
SN - 0819409146
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 333
EP - 340
BT - Proceedings of SPIE - The International Society for Optical Engineering
PB - Publ by Int Soc for Optical Engineering
T2 - Soft X-Ray Microscopy
Y2 - 19 July 1992 through 21 July 1992
ER -