ROSALIND: Rapid Detection of Chemical Contaminants with In Vitro Transcription Factor-Based Biosensors

Jaeyoung K. Jung, Khalid K. Alam, Julius B. Lucks*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

ROSALIND (RNA Output Sensors Activated by Ligand Induction) is an in vitro biosensing system that detects small molecules using regulated transcription reactions. It consists of three key components: (1) RNA polymerases, (2) allosteric protein transcription factors, and (3) synthetic DNA transcription templates that together regulate the synthesis of a fluorescence-activating RNA aptamer. The system can detect a wide range of chemicals including antibiotics, small molecules, and metal ions. We have demonstrated that ROSALIND can be lyophilized and transported at ambient conditions for water testing on-site. Here, we describe how to set up a ROSALIND reaction for detecting various chemical contaminants in water using a model transcription factor as well as how to build a new ROSALIND sensor.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages325-342
Number of pages18
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2433
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Allosteric transcription factors
  • Biosensors
  • Cell-free synthetic biology
  • In vitro transcription
  • RNA aptamer
  • Water quality monitoring

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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