S100B Secretion in Acute Brain Slices: Modulation by Extracellular Levels of Ca2+ and K+

Patrícia Nardin, Lucas Tortorelli, André Quincozes-Santos, Lúcia Maria V De Almeida, Marina C. Leite, Ana Paula Thomazi, Carmem Gottfried, Susana T. Wofchuk, Rosario Donato, Carlos Alberto Gonçalves*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Hippocampal slices have been widely used to investigate electrophysiological and metabolic neuronal parameters, as well as parameters of astroglial activity including protein phosphorylation and glutamate uptake. S100B is an astroglial-derived protein, which extracellularly plays a neurotrophic activity during development and excitotoxic insult. Herein, we characterized S100B secretion in acute hippocampal slices exposed to different concentrations of K+ and Ca2+ in the extracellular medium. Absence of Ca2+ and/or low K+ (0.2 mM KCl) caused an increase in S100B secretion, possibly by mobilization of internal stores of Ca2+. In contrast, high K+ (30 mM KCl) or calcium channel blockers caused a decrease in S100B secretion. This study suggests that exposure of acute hippocampal slices to low- and high-K+ could be used as an assay to evaluate astrocyte activity by S100B secretion: positively regulated by low K+ (possibly involving mobilization of internal stores of Ca2+) and negatively regulated by high-K+ (likely secondary to influx of K+).

Original languageEnglish (US)
Pages (from-to)1603-1611
Number of pages9
JournalNeurochemical Research
Volume34
Issue number9
DOIs
StatePublished - Sep 2009

Keywords

  • Astrocyte
  • Brain slice
  • Hippocampus
  • K-Depolarisation
  • S100B Secretion

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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