@inbook{b258a7b875cd4b358f7f63c8b0818960,
title = "Scalable CRISPR Screens in Zebrafish Using MIC-Drop",
abstract = "CRISPR-Cas9 is a powerful tool to interrogate gene function in a targeted and systematic manner. Although the technology has been scaled up for large-scale genetic screens in cell culture, similar scale screens in vivo have been extremely challenging due to the cost, labor, and time required to generate and keep track of thousands of mutant animals. We reported the development of Multiplexed Intermixed CRISPR Droplets (MIC-Drop), a platform that makes large-scale reverse genetic screens possible in zebrafish. In this chapter, we provide a detailed protocol to conduct large-scale genetic screens using this novel platform.",
keywords = "CRISPR-Cas9, F0 CRISPR screen, Genetic screen, High-throughput, In vivo, Reverse genetics, Zebrafish",
author = "Saba Parvez and Tejia Zhang and Peterson, {Randall T.}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3401-1_21",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "319--334",
booktitle = "Methods in Molecular Biology",
}