Scavenger receptors mediate cellular uptake of polyvalent oligonucleotide-functionalized gold nanoparticles

Pinal C. Patel, David A. Giljohann, Weston L. Daniel, Dan Zheng, Andrew E. Prigodich, Chad A. Mirkin

Research output: Contribution to journalArticle

215 Scopus citations

Abstract

Mammalian cells have been shown to internalize oligonucleotide- functionalized gold nanoparticles (DNA-Au NPs or siRNA-Au NPs) without the aid of auxiliary transfection agents and use them to initiate an antisense or RNAi response. Previous studies have shown that the dense monolayer of oligonucleotides on the nanoparticle leads to the adsorption of serum proteins and facilitates cellular uptake. Here, we show that serum proteins generally act to inhibit cellular uptake of DNA-Au NPs. We identify the pathway for DNA-Au NP entry in HeLa cells. Biochemical analyses indicate that DNA-Au NPs are taken up by a process involving receptor-mediated endocytosis. Evidence shows that DNA-Au NP entry is primarily mediated by scavenger receptors, a class of pattern-recognition receptors. This uptake mechanism appears to be conserved across species, as blocking the same receptors in mouse cells also disrupted DNA-Au NP entry. Polyvalent nanoparticles functionalized with siRNA are shown to enter through the same pathway. Thus, scavenger receptors are required for cellular uptake of polyvalent oligonucleotide functionalized nanoparticles.

Original languageEnglish (US)
Pages (from-to)2250-2256
Number of pages7
JournalBioconjugate Chemistry
Volume21
Issue number12
DOIs
StatePublished - Dec 15 2010

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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