Screening for occult nodal metastasis in squamous cell carcinoma of the vulva

Charles M. Leys, Ellen M. Hartenbach*, Gholam Reza Hafez, David M. Mahvi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Metastases to inguinofemoral lymph nodes in patients with carcinoma of the vulva alter the prognosis and treatment of this disease. Our goal was to determine if immunohistochemical staining could reveal occult metastatic nodal disease not detected with routine hematoxylin and eosin staining. We retrospectively examined a total of 110 lymph nodes from 10 patients who had undergone lymph node dissection and found to have all negative node. Paraffin embedded lymph nodes were immunostained with a monoclonal antibody directed against multiple low- and high molecular weight cytokeratins. Micrometastases were not detected in any lymph nodes examined with immunohistochemistry. All positive and negative controls yielded satisfactory results. It is concluded that immunohistochemistry with cytokeratin antibodies does not provide greater sensitivity than routine hematoxylin and eosin staining for the detection of nodal metastases in vulvar carcinoma.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalInternational Journal of Gynecological Pathology
Issue number3
StatePublished - Jan 1 2000


  • Cytokeratin
  • Immunohistochemistry
  • Occult metastasis
  • Vulvar carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Obstetrics and Gynecology


Dive into the research topics of 'Screening for occult nodal metastasis in squamous cell carcinoma of the vulva'. Together they form a unique fingerprint.

Cite this