Seeing beyond the tip of the iceberg: A deep analysis of the venome of the Brazilian Rattlesnake, Crotalus durissus terrificus

Rafael D. Melani, Gabriel D.T. Araujo, Paulo C. Carvalho, Livia Goto, Fábio C.S. Nogueira, Magno Junqueira, Gilberto B. Domont*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The complete characterization of the snake venom protein components is a requirement for a systems-wide understanding of their biological context. In this work, we provide a deep proteomic characterization of Crotalus durissus terrificus venom using different bottom-up approaches. We identified more than five times more protein families than the sum of all identifications previously reported. For the first time in this sub-species, we report the identification of three new toxin families: CRISP, phospholipase-B, and SVVEGF. This work also describes proteins involved in regulation of toxin synthesis and processing that are present in venom.

Original languageEnglish (US)
Pages (from-to)144-156
Number of pages13
JournalEuPA Open Proteomics
Volume8
DOIs
StatePublished - Sep 1 2015

Keywords

  • Bottom-up approaches
  • CPLL
  • Crotalus durissus terrificus venom
  • IEF
  • In-solution digestion
  • Shotgun

ASJC Scopus subject areas

  • Biochemistry

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