Segmentation of PET images for computer-aided functional quantification of tuberculosis in small animal models

Brent Foster, Ulas Bagci*, Ziyue Xu, Bappaditya Dey, Brian Luna, William Bishai, Sanjay Jain, Daniel J. Mollura

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


Pulmonary infections often cause spatially diffuse and multi-focal radiotracer uptake in positron emission tomography (PET) images, which makes accurate quantification of the disease extent challenging. Image segmentation plays a vital role in quantifying uptake due to the distributed nature of immuno-pathology and associated metabolic activities in pulmonary infection, specifically tuberculosis (TB). For this task, thresholding-based segmentation methods may be better suited over other methods; however, performance of the thresholding-based methods depend on the selection of thresholding parameters, which are often suboptimal. Several optimal thresholding techniques have been proposed in the literature, but there is currently no consensus on how to determine the optimal threshold for precise identification of spatially diffuse and multi-focal radiotracer uptake. In this study, we propose a method to select optimal thresholding levels by utilizing a novel intensity affinity metric within the affinity propagation clustering framework. We tested the proposed method against 70 longitudinal PET images of rabbits infected with TB. The overall dice similarity coefficient between the segmentation from the proposed method and two expert segmentations was found to be 91.25 ± 8.01% with a sensitivity of 88.80 ± 12.59% and a specificity of 96.01 ± 9.20%. High accuracy and heightened efficiency of our proposed method, as compared to other PET image segmentation methods, were reported with various quantification metrics.

Original languageEnglish (US)
Article number6655914
Pages (from-to)711-724
Number of pages14
JournalIEEE Transactions on Biomedical Engineering
Issue number3
StatePublished - Mar 2014
Externally publishedYes


  • Affinity propagation
  • image segmentation
  • infectious diseases
  • nuclear medicine
  • positron emission tomography (PET)
  • radiology
  • small animal models
  • tuberculosis (TB)

ASJC Scopus subject areas

  • Biomedical Engineering


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