Selective cleavage in the avian retroviral long terminal repeat sequence by the endonuclease associated with the αβ form of avian reverse transcriptase

G. Duyk, J. Leis, M. Longiaru, A. M. Skalka

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37 Scopus citations

Abstract

M13 recombinant DNA clones containing a 350-base sequence derived from the EcoRI fragment of two tandemly linked Rous-associated virus 2 (RAV-2) long terminal repeat (LTR) sequences have been used to map reverse transcriptase-associated endonuclease (RT-endonuclease) cleavage sites by primer extension studies. Under appropriate conditions, the αβ form of RT-endonuclease (composed of both the α and β subunits) purified from Avian sarcoma virus (Pr-C and B-77 strains) introduces a specific break in the inverted complementary repeat sequence found at the junction of the LTRs. The cleavage sites occur in the same nucleotide sequence in (-) and (+) DNA strands; together they have the potential of generating a 6-base-pair staggered overlap that spans the junction. This supports the notion that the enzyme is involved in viral DNA integration. Other RT-endonuclease sites were analyzed. A second site, which occurs in the lac region of the M13 vector DNA upstream from the unique EcoRI cloning site, bears no apparent sequence homology to the site at the junction of the LRTs. However, it also lies within an inverted complementary repeat and, as is the case for the site in the LTR, the break occurs to the 5' side of the axis of symmetry. Cleavage at this second site is suppressed when the vector contains the RAV-2 LTR insert. Thus, the viral LTR appears to exert a cis effect that can influence a region over 300 base pairs away.

Original languageEnglish (US)
Pages (from-to)6745-6749
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number22 I
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • General

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