Selective expansion of skeletal muscle stem cells from bulk muscle cells in soft three-dimensional fibrin gel

Pei Zhu, Yalu Zhou, Furen Wu, Yuanfan Hong, Xin Wang, Gajendra Shekhawat, Jeffrey Mosenson, Wen Shu Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three-dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell-based therapies for various muscle disorders.

Original languageEnglish (US)
Pages (from-to)1412-1423
Number of pages12
JournalStem Cells Translational Medicine
Volume6
Issue number5
DOIs
StatePublished - May 2017

Keywords

  • Muscle stem cells
  • Myogenic progenitor cells
  • Satellite cells
  • Three-dimensional fibrin gel

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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