Abstract
Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three-dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell-based therapies for various muscle disorders.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1412-1423 |
| Number of pages | 12 |
| Journal | Stem Cells Translational Medicine |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2017 |
Funding
We thank members of the WU laboratory for support. We also thank the Research Resources Center Flow Cytometry Service at UIC for cell analysis and sorting, Dr. G. Adami and L. Adami for their critical reading of the manuscript. This work made use of the EPIC facility of the NUANCE Center at Northwestern University, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF NNCI-1542205); the MRSEC program (NSF DMR-1121262) at the Materials Research Center; the International Institute for Nanotechnology (IIN); the Keck Foundation; and the State of Illinois, through the IIN. This work was supported by NIA/NIH grant (R01AG040182) and NIDDK/NIH grant (R01DK090478).
Keywords
- Muscle stem cells
- Myogenic progenitor cells
- Satellite cells
- Three-dimensional fibrin gel
ASJC Scopus subject areas
- General Medicine