Selective neurofilament (SMI-32, FNP-7 and N200) expression in subpopulations of layer V pyramidal neurons In Vivo and In Vitro

Courtney C.J. Voelker, Nathalie Garin, Jeremy S.H. Taylor, Beat H. Gähwiler, Jean Pierre Hornung, Zoltán Molnár*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

There are two main types of layer V pyramidal neurons in rat cortex. Type I neurons have tufted apical dendrites extending into layer I, produce bursts of action potentials and project to subcortical targets (spinal cord, superior colliculus and pontine nuclei). Type II neurons have apical dendrites, which arborize in layers II-IV, do not produce bursts of action potentials and project to ipsilateral and contralateral cortex. The specific expression of different genes and proteins in these two distinct layer V neurons is unknown. To distinguish between distinct subpopulations, fluorescent microspheres were injected into subcortical targets (labeling type I neurons) or primary somatosensory cortex (labeling type II neurons) of adult rats. After transport, cortical sections were processed for immunohistochemistry using various antibodies. This study demonstrated that antigens recognized by SM1-32, N200 and FNP-7 antibodies were only expressed in subcortical (type I)-but not in contralateral (type II) -projecting neurons. NR1, NR2a/b, PLCβ1, BDNF, NGF and TrkB antigens were highly expressed in all neuronal subpopulations examined. Organotypic culture experiments demonstrated that the development of neurofilament expression and laminar specificity does not depend on the presence of the subcortical targets. This study suggests specific markers for the subcortical projecting layer V neuron subpopulations.

Original languageEnglish (US)
Pages (from-to)1276-1286
Number of pages11
JournalCerebral Cortex
Volume14
Issue number11
DOIs
StatePublished - Nov 2004
Externally publishedYes

Keywords

  • Cell differentiation
  • Corpus callosum
  • Intracortical and intercortical connections
  • Spinal cord
  • Superior colliculus

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

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