Selenoprotein P regulates 1-(4-Chlorophenyl)-benzo-2,5-quinone-induced oxidative stress and toxicity in human keratinocytes

Wusheng Xiao, Yueming Zhu, Ehab H. Sarsour, Amanda L. Kalen, Nukhet Aykin-Burns, Douglas R. Spitz, Prabhat C. Goswami*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


Polychlorinated biphenyls and their metabolites are environmental pollutants that are believed to have adverse health effects presumably by inducing oxidative stress. To determine if 1-(4-Chlorophenyl)-benzo-2,5-quinone (4-ClBQ; metabolite of 4-monochlorobiphenyl, PCB3)-induced oxidative stress is associated with changes in the expression of specific antioxidant genes, mRNA levels of 92 oxidative stress-response genes were analyzed using TaqMan Array Human Antioxidant Mechanisms (Life Technologies), and results were verified by performing quantitative RT-PCR assays. The expression of selenoprotein P (sepp1) was significantly downregulated (8- to 10-fold) in 4-ClBQ-treated HaCaT human skin keratinocytes, which correlated with a significant increase in MitoSOX oxidation. Overexpression of Mn-superoxide dismutase or catalase or treatment with N-acetyl-l-cysteine suppressed 4-ClBQ-induced toxicity. Sodium selenite supplementation also suppressed 4-ClBQ-induced decrease in sepp1 expression, which was associated with a significant inhibition in cell death. Furthermore, HaCaT cells overexpressing sepp1 were resistant to 4-ClBQ-induced oxidative stress and toxicity. These results demonstrate that SEPP1 represents a previously unrecognized regulator of PCB-induced biological effects. These results support the speculation that selenoproteins can be an attractive countermeasure for PCB-induced adverse biological effects.

Original languageEnglish (US)
Pages (from-to)70-77
Number of pages8
JournalFree Radical Biology and Medicine
StatePublished - 2013


  • 4-ClBQ
  • Free radicals
  • HaCaT
  • Oxidative stress
  • PCB3
  • Polychlorinated biphenyls
  • Selenoprotein P

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)


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