Self-assembled monolayer facilitates epithelial-Mesenchymal interactions mimicking odontogenesis

Tanvi Muni, Milan Mrksich, Anne George*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Cell-cell interactions are vital for embryonic organ development and normal function of differentiated cells and tissues. In this study we have developed a self-assembled monolayer-based co-culture system to study tooth morphogenesis. Specifically, we designed a 2-D microenvironment present in the dental tissue by creating a well-structured, laterally organized epithelial and mesenchymal cell co-culture system by patterning the cell-attachment substrate. Chemical modifications were used to develop tunable surface patterns to facilitate epithelial-mesenchymal interactions mimicking the developing tooth. Such a design promoted interactions between monolayer's of the 2 cell types and provided signaling cues that resulted in cellular differentiation and mineralized matrix formation. Gene expression analysis showed that these co-cultures mimicked in-vivo conditions than monolayer cultures of a single cell type.

Original languageEnglish (US)
Pages (from-to)26-33
Number of pages8
JournalConnective tissue research
Issue number1
StatePublished - Jan 2014


  • Co-culture
  • Dentin
  • Epithelial-mesenchymal interactions
  • Mineralized matrix
  • Self-assembled monolayer
  • Tooth development

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology


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