Abstract
The efficiency of biomaterial-based gene delivery is determined by the interaction of the material and the vector. For lipoplexes, surface immobilization has been used to transfect cells for applications such as cell arrays and model tissue formation through patterned transfection. Further increases in the delivery efficiency are limited by cellular internalization, which may be overcome by altering the material/vector interactions. In this report, we investigated the modification of the lipoplex physical properties through self-assembly with cationic peptides, and subsequently quantified cellular association, internalization and nuclear accumulation of DNA and transfection. Relative to lipid alone, peptide-lipoplexes enhanced transfection by up to 4.6-fold. The presence of the peptide in the lipoplex increased internalization efficiency by up to 4.5-fold, decreased the percentage of lysosomal DNA by 2.1-fold and increased the efficiency of nuclear accumulation by 3.0-fold. In addition, an analysis of internalization pathways for peptide-lipoplexes indicated a greater role of clathrin and caveolae-mediated endocytosis relative to macropinocytois, which was not observed for peptide-free lipoplexes. These results demonstrate peptide-induced enhancement of gene transfer by surface immobilization due to increased cellular internalization and nuclear accumulation, which has numerous applications ranging from cell-based assays to regenerative medicine.
Original language | English (US) |
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Pages (from-to) | 903-912 |
Number of pages | 10 |
Journal | Acta Biomaterialia |
Volume | 5 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1 2009 |
Funding
Support for this research was provided in part by the NIH (R01 EB003806-01 (A.E.B., L.D.S.) and R01 GM066830 (L.D.S.)), the Institute for BioNanotechnology in Medicine (IBNAM) at Northwestern University, and a Ford Foundation Predoctoral Fellowship (J.C.R.). Confocal microscopy images were obtained at the Biological Imaging Facility (BIF) at Northwestern University. ESI and MALDI-TOF mass spectrometry were performed at the Analytical Services Laboratory at Northwestern University. We would like to thank William Russin for technical assistance.
Keywords
- Gene delivery
- Lipoplex
- Peptide
- Self-assembly
- Substrate-mediated
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
- Biotechnology
- Biochemistry
- Molecular Biology