Self-directing optimization of parameters for extracellular chitinase production by Trichoderma harzianum in batch mode

P. Arthur Felse, T. Panda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

A novel optimization procedure has been developed to determine the best combination of variables in large scale fermentation systems. The self- directing optimization or the rotating simplex method of optimization was employed to determine the best suitable combination of parameters, pH (controlled), aeration rate and agitation rate for maximal production of chitinase by Trichoderma harzianum in batch culture. Extracellular enzyme activity increased from an initial value of 0.109 U under unoptimized conditions to a final value of 0.384 U after optimization. A controlled pH of 4.9, an aeration rate of 1.51 l-1 per min, and an agitation rate of 224 rpm was found to be the best combination.

Original languageEnglish (US)
Pages (from-to)563-566
Number of pages4
JournalProcess Biochemistry
Volume34
Issue number6-7
DOIs
StatePublished - Sep 1 1999

Keywords

  • Bioreactor
  • Chitinase
  • Non-statistical
  • Self-directing optimization
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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