TY - JOUR
T1 - SEM-dissection of a human embryo derived from an ectopic pregnancy
AU - Hendrix, Mary J.C.
AU - Brailey, Jacqueline L.
AU - Shenker, Lewis
N1 - Funding Information:
The authors wish to thank Dr. Jay B. Angevine, Jr for his editorial advice with the manuscript. Also, the authors are grateful to Dr. Ronan O’Rahilly, Carnegie Laboratories of Embryology, Davis, CA, for his interpretation of one of the micrographs presented in this paper. The research was supported by NIH Grant R01 HL 28850-02 and an American Heart Association grant.
PY - 1985/5
Y1 - 1985/5
N2 - A 6.5-week-old human embryo with an approximate crown-rump length of 13.2 mm was obtained from a tubal pregnancy. Two hours before surgical removal, the embryo was imaged with real time ultrasound and was noted to have rhythmic cardiac motion. Subsequent to surgical removal, the embryo was dissected free from the placenta and prepared for routine scanning electron microscopic (SEM) studies. Progressive stages of dissection with microsurgical instruments followed by SEM photography elucidated the three-dimensional aspects of embryonic development of many structures, including the lens placode, tongue bud, Rathke's pouch, atrial and ventricular foramina, primitive intestinal loop and undifferentiated external genitalia. Almost certainly, such clear views of dissected structures can contribute to our understanding of human embryonic development.
AB - A 6.5-week-old human embryo with an approximate crown-rump length of 13.2 mm was obtained from a tubal pregnancy. Two hours before surgical removal, the embryo was imaged with real time ultrasound and was noted to have rhythmic cardiac motion. Subsequent to surgical removal, the embryo was dissected free from the placenta and prepared for routine scanning electron microscopic (SEM) studies. Progressive stages of dissection with microsurgical instruments followed by SEM photography elucidated the three-dimensional aspects of embryonic development of many structures, including the lens placode, tongue bud, Rathke's pouch, atrial and ventricular foramina, primitive intestinal loop and undifferentiated external genitalia. Almost certainly, such clear views of dissected structures can contribute to our understanding of human embryonic development.
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U2 - 10.1016/0378-3782(85)90119-7
DO - 10.1016/0378-3782(85)90119-7
M3 - Article
C2 - 4006825
AN - SCOPUS:0021843296
SN - 0378-3782
VL - 11
SP - 61
EP - 68
JO - Early Human Development
JF - Early Human Development
IS - 1
ER -