The application of staining with Coomassie brilliant blue in isopropanol acetic acid to the quantitation of cyanogen bromide peptides of collagen on electrophoresis in SDS-polyacrylamide gels was evaluated. A linear relationship between stain uptake and weight of peptide material loaded onto the gel was demonstrated over the range investigated (up to 40 μg of total mixture of peptides) for each component resolved. The larger peptides (≥18,000 MW) could be quantitated either as individual components or as a proportion of the total mixture to a precision of ±15% or better on quantities of 1 μg or less per band.
ASJC Scopus subject areas
- Molecular Biology