Semitransparent nanostructured films for imaging mass spectrometry and optical microscopy

Jay G. Forsythe, Joshua A. Broussard, Jenifer L. Lawrie, Michal Kliman, Yang Jiao, Sharon M. Weiss*, Donna J. Webb, John A. McLean

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Semitransparent porous silicon substrates have been developed for pairing nanostructure-initiator mass spectrometry (NIMS) imaging with traditional optical-based microscopy techniques. Substrates were optimized to generate the largest NIMS signal while maintaining sufficient transparency to allow visible light to pass through for optical microscopy. Using these substrates, both phase-contrast and NIMS images of phospholipids from a scratch-wounded cell monolayer were obtained. NIMS images were generated using a spatial resolution of 14 μm. Coupled with further improvements in spatial resolution, this approach may allow for the localization of intact biological molecules within cells without the need for labeling.

Original languageEnglish (US)
Pages (from-to)10665-10670
Number of pages6
JournalAnalytical Chemistry
Volume84
Issue number24
DOIs
StatePublished - Dec 18 2012

ASJC Scopus subject areas

  • Analytical Chemistry

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