Sensitive Spectrophotometer for Measuring Kinetics of Reversible Systems

P. A. Loach; R. J. Loyd

doi:10.1021/ac60244a020

Sensitive Spectrophotometer for Measuring Kinetics of Reversible Systems

P. A. Loach, R. J. Loyd

Molecular Biosciences

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16 Scopus citations

Abstract

Details are given for construction of a sensitive spectrophotometer whose components include a double monochromator for achieving a high spectral purity in the detecting beam and a feedback circuit for stabilizing the light emitted from the detecting beam source. The instrument is capable of quantitatively measuring changes as small as 5×10-5 absorbance unit, has a resolution of 1 A. over much of the spectrum (2600 to 12000 A.), and can measure precisely kinetic parameters which may vary from 10-4 second to minutes. Typical light-induced absorbance changes for the photosynthetic bacterium Rhodospirillum rubrum are presented, and the importance of using a low intensity detecting beam for such systems is underscored.

Original language	English (US)
Pages (from-to)	1709-1713
Number of pages	5
Journal	Analytical Chemistry
Volume	38
Issue number	12
DOIs	https://doi.org/10.1021/ac60244a020
State	Published - Jan 1 1966

ASJC Scopus subject areas

Analytical Chemistry

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AB - Details are given for construction of a sensitive spectrophotometer whose components include a double monochromator for achieving a high spectral purity in the detecting beam and a feedback circuit for stabilizing the light emitted from the detecting beam source. The instrument is capable of quantitatively measuring changes as small as 5×10-5 absorbance unit, has a resolution of 1 A. over much of the spectrum (2600 to 12000 A.), and can measure precisely kinetic parameters which may vary from 10-4 second to minutes. Typical light-induced absorbance changes for the photosynthetic bacterium Rhodospirillum rubrum are presented, and the importance of using a low intensity detecting beam for such systems is underscored.

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Sensitive Spectrophotometer for Measuring Kinetics of Reversible Systems

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