Sensitivity enhancement by exchange mediated magnetization transfer of the xenon biosensor signal

Sandra Garcia; Lana Chavez; Thomas J. Lowery; Song I. Han; David E. Wemmer; Alexander Pines

doi:10.1016/j.jmr.2006.09.010

Sensitivity enhancement by exchange mediated magnetization transfer of the xenon biosensor signal

Sandra Garcia, Lana Chavez, Thomas J. Lowery, Song I. Han, David E. Wemmer, Alexander Pines^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Hyperpolarized xenon associated with ligand derivatized cryptophane-A cages has been developed as a NMR based biosensor. To optimize the detection sensitivity we describe use of xenon exchange between the caged and bulk dissolved xenon as an effective signal amplifier. This approach, somewhat analogous to 'remote detection' described recently, uses the chemical exchange to repeatedly transfer spectroscopic information from caged to bulk xenon, effectively integrating the caged signal. After an optimized integration period, the signal is read out by observation of the bulk magnetization. The spectrum of the caged xenon is reconstructed through use of a variable evolution period before transfer and Fourier analysis of the bulk signal as a function of the evolution time.

Original language	English (US)
Pages (from-to)	72-77
Number of pages	6
Journal	Journal of Magnetic Resonance
Volume	184
Issue number	1
DOIs	https://doi.org/10.1016/j.jmr.2006.09.010
State	Published - Jan 2007

Keywords

Biosensor
Chemical exchange
Remote detection
Xenon

ASJC Scopus subject areas

Biophysics
Biochemistry
Nuclear and High Energy Physics
Condensed Matter Physics

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10.1016/j.jmr.2006.09.010

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title = "Sensitivity enhancement by exchange mediated magnetization transfer of the xenon biosensor signal",

abstract = "Hyperpolarized xenon associated with ligand derivatized cryptophane-A cages has been developed as a NMR based biosensor. To optimize the detection sensitivity we describe use of xenon exchange between the caged and bulk dissolved xenon as an effective signal amplifier. This approach, somewhat analogous to 'remote detection' described recently, uses the chemical exchange to repeatedly transfer spectroscopic information from caged to bulk xenon, effectively integrating the caged signal. After an optimized integration period, the signal is read out by observation of the bulk magnetization. The spectrum of the caged xenon is reconstructed through use of a variable evolution period before transfer and Fourier analysis of the bulk signal as a function of the evolution time.",

keywords = "Biosensor, Chemical exchange, Remote detection, Xenon",

author = "Sandra Garcia and Lana Chavez and Lowery, {Thomas J.} and Han, {Song I.} and Wemmer, {David E.} and Alexander Pines",

note = "Funding Information: We thank Josef Granwehr for many hours of useful discussions. S.G. acknowledges ITRI for funding. T.J.L. acknowledges UC BREP for a training grant. This work was supported by the Director of the Office of Science (Materials Sciences and Physical Biosciences Divisions of the U.S. Department of Energy under the Contract DE-AC03-76SF00098).",

year = "2007",

month = jan,

doi = "10.1016/j.jmr.2006.09.010",

language = "English (US)",

volume = "184",

pages = "72--77",

journal = "Journal of Magnetic Resonance",

issn = "1090-7807",

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T1 - Sensitivity enhancement by exchange mediated magnetization transfer of the xenon biosensor signal

AU - Garcia, Sandra

AU - Chavez, Lana

AU - Lowery, Thomas J.

AU - Han, Song I.

AU - Wemmer, David E.

AU - Pines, Alexander

N1 - Funding Information: We thank Josef Granwehr for many hours of useful discussions. S.G. acknowledges ITRI for funding. T.J.L. acknowledges UC BREP for a training grant. This work was supported by the Director of the Office of Science (Materials Sciences and Physical Biosciences Divisions of the U.S. Department of Energy under the Contract DE-AC03-76SF00098).

PY - 2007/1

Y1 - 2007/1

N2 - Hyperpolarized xenon associated with ligand derivatized cryptophane-A cages has been developed as a NMR based biosensor. To optimize the detection sensitivity we describe use of xenon exchange between the caged and bulk dissolved xenon as an effective signal amplifier. This approach, somewhat analogous to 'remote detection' described recently, uses the chemical exchange to repeatedly transfer spectroscopic information from caged to bulk xenon, effectively integrating the caged signal. After an optimized integration period, the signal is read out by observation of the bulk magnetization. The spectrum of the caged xenon is reconstructed through use of a variable evolution period before transfer and Fourier analysis of the bulk signal as a function of the evolution time.

AB - Hyperpolarized xenon associated with ligand derivatized cryptophane-A cages has been developed as a NMR based biosensor. To optimize the detection sensitivity we describe use of xenon exchange between the caged and bulk dissolved xenon as an effective signal amplifier. This approach, somewhat analogous to 'remote detection' described recently, uses the chemical exchange to repeatedly transfer spectroscopic information from caged to bulk xenon, effectively integrating the caged signal. After an optimized integration period, the signal is read out by observation of the bulk magnetization. The spectrum of the caged xenon is reconstructed through use of a variable evolution period before transfer and Fourier analysis of the bulk signal as a function of the evolution time.

KW - Biosensor

KW - Chemical exchange

KW - Remote detection

KW - Xenon

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SP - 72

EP - 77

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

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Sensitivity enhancement by exchange mediated magnetization transfer of the xenon biosensor signal

Abstract

Keywords

ASJC Scopus subject areas

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