Separation of tricomponent protein mixtures with triblock nanorods

Byung Keun Oh, Sungho Park, Jill E. Millstone, Seung Woo Lee, Ki Bum Lee, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


Two-component triblock magnetic nanorods with gold end blocks and nickel interior blocks have been synthesized and used as affinity templates for the simultaneous and efficient separation of a three-component protein mixture. The gold blocks were selectively functionalized with 11-amino-1-undecanethiol, and then glutaraldehyde was used to covalently attach nitrostreptavidin to them. His-tagged proteins bind to the nickel block and biotin-tagged proteins bind to the functionalized gold ends, allowing one to separate a mixture of three proteins with a single material. Each surface-bound protein can be released selectively using imidazole for the His-tagged protein and biotin for the biotinylated protein.

Original languageEnglish (US)
Pages (from-to)11825-11829
Number of pages5
JournalJournal of the American Chemical Society
Issue number36
StatePublished - Sep 13 2006

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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