Sequence and identification of the nucleotide binding site for the elongation factor tu from Thermus thermophilus HB8

Lothar Seidler*, Marcus Peter, Franz Meissner, Mathias Sprinzl

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Two structural genes for the Thermus thermophilus elongation factor Tu (tuf) were identified by cross-hybridization with the tufA gene from E.coli. The sequence of one of these tuf genes, localized on a 6.6 kb Bam HI fragment, was determined and confirmed by partial protein sequencing of an authentic elongation factor Tu from T. thermophilus HB8. Expression of this tuf gene in E.coli minicells provided a low amount of immuno-precipitable thermophilic EF-Tu. Affinity labeling of the T. thermophilus EF-Tu and sequence comparison with homologous proteins from other organisms were U.S. to identify the gusnosine-nucleotide binding domain.

Original languageEnglish (US)
Pages (from-to)9263-9277
Number of pages15
JournalNucleic acids research
Volume15
Issue number22
DOIs
StatePublished - Nov 25 1987

ASJC Scopus subject areas

  • Genetics

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