Sequence characterization of the membrane protein gene of paramyxovirus simian virus 5

Hooshmand Sheshberadaran, Roberta A. Lamb*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The complete nucleotide sequence of the membrane (M) protein gene of the paramyxovirus simian virus 5 (SV5) was determined from cDNA clones of viral mRNAs. The M gene boundaries were determined by (i) primer extension sequencing on M mRNA; (ii) nuclease S1 analysis; and (iii) primer extension sequencing on viral genomic RNA. The M gene mRNA consisted of 1371 templated nucleotides. It contains a single large open reading frame that can encode a protein of 377 amino acids with a predicted Mr = 42,253. The authenticity of the predicted M protein coding sequence was confirmed by synthesis of the M protein from mRNA synthesized from cDNA. The predicted M amino acid sequence indicated it is an overall hydrophobic protein carrying a net positive charge. Alignment of the SV5 protein amino acid sequence with the M protein sequences of other paramyxoviruses indicated that these viruses fall into the following two groups: (1) SV5, mumps virus, and Newcastle disease virus; or (2) Sendai, parainfluenza virus type 3, measles virus, and canine distemper virus, with mumps virus M sequence being the most closely related to SV5.

Original languageEnglish (US)
Pages (from-to)234-243
Number of pages10
JournalVirology
Volume176
Issue number1
DOIs
StatePublished - May 1990

ASJC Scopus subject areas

  • Virology

Fingerprint

Dive into the research topics of 'Sequence characterization of the membrane protein gene of paramyxovirus simian virus 5'. Together they form a unique fingerprint.

Cite this