Sequential histologic study of rat liver during peroxisome proliferator [4-Chloro-6-(2, 3-xylidino)-2-pyrimidinylthio]acetic Acid (Wy-14, 643)-lnduced carcinogenesi

M. Sambaslva Rao, Narendra D. Lalwanl, Janardan K. Reddy

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

The livers of male inbred F344 rats fed Wy-14, 643 [4-chloro-6-(2, 3-xylidino)-2-pyrimidinylthiol acetic acid (CAS: 50892-23-4) in the diet at a concentration of 0.1% (wt/wt) were examined sequentially at 5, 10, 17, 21, 26, 30, 35, 40, 52, 60, and 70 weeks. At 5 weeks the livers were markedly enlarged and histologically showed markedly enlarged hepatocytes with prominent nucleoli. At 21 weeks altered acidophilic areas were seen in 2 of 3 animals that were killed. Between 26 and 52 weeks neoplastic nodules were noted of 1–8 mm containing cells with morphologic features similar to those observed in altered areas. Hepatocellular carcinomas (HCC) were observed in 1 animal killed at 30 weeks and in all the animals sacrificed at 60 weeks and later. [3H]thymidine nuclear labeling studies showed marked proliferative activity of cells in altered areas, neoplastic nodules, and HCC. Altered areas, neoplastic nodules, and HCC were consistently negative for γ-glutamyltransferase and showed decreased ATPase activity. Glucose-6-phosphatase (Glc-6-Pase) activity was decreased in altered areas and neoplastic nodules. However, some of the HCC showed a strong positive reaction for Glc-6-Pase.

Original languageEnglish (US)
Pages (from-to)983-990
Number of pages8
JournalJournal of the National Cancer Institute
Volume73
Issue number4
DOIs
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Sequential histologic study of rat liver during peroxisome proliferator [4-Chloro-6-(2, 3-xylidino)-2-pyrimidinylthio]acetic Acid (Wy-14, 643)-lnduced carcinogenesi'. Together they form a unique fingerprint.

Cite this