Serotonin enhances excitability in neostriatal neurons by reducing voltage-dependent potassium currents

Alessandro Stefani; D. James Surmeier; S. T. Kitai

doi:10.1016/0006-8993(90)90851-2

Serotonin enhances excitability in neostriatal neurons by reducing voltage-dependent potassium currents

Alessandro Stefani, D. James Surmeier, S. T. Kitai^*

^*Corresponding author for this work

Neuroscience

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The physiological effects of serotonin (5-HT) on rat neostriatal neurons were investigated using current-clamp techniques in neostriatal slices and voltage-clamp techniques in acutely dissociated adult neostriatal neurons. In most neurons (35/51), bath-applied 5-HT (10-60 gmM) decreased the first spike latency and increased the evoked firing frequency. Membrane input resistance was also increased in most neurons (35/35) but could not explain the enhanced responsiveness. Tetrodotoxin, at concentrations sufficient to block spike production, did not block the ability of 5-HT to enhance the slow ramp-like voltage trajectory produced by depolarizing current injection. The role of potassium currents in the 5-HT effect was examined using whole cell voltage-clamp; in 6 of 9 neurons, 5-HT reversibly decreased inactivating potassium currents activated by depolarization. These experiments suggest that 5-HT's effect on the ramp trajectory may be mediated by a reduction of potassium currents activated by sub-threshold depolarization.

Original language	English (US)
Pages (from-to)	354-357
Number of pages	4
Journal	Brain research
Volume	529
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(90)90851-2
State	Published - Oct 8 1990

Keywords

Modulation
Neostriatum
Potassium current
Serotonin
Voltage-clamp

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(90)90851-2

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title = "Serotonin enhances excitability in neostriatal neurons by reducing voltage-dependent potassium currents",

abstract = "The physiological effects of serotonin (5-HT) on rat neostriatal neurons were investigated using current-clamp techniques in neostriatal slices and voltage-clamp techniques in acutely dissociated adult neostriatal neurons. In most neurons (35/51), bath-applied 5-HT (10-60 gmM) decreased the first spike latency and increased the evoked firing frequency. Membrane input resistance was also increased in most neurons (35/35) but could not explain the enhanced responsiveness. Tetrodotoxin, at concentrations sufficient to block spike production, did not block the ability of 5-HT to enhance the slow ramp-like voltage trajectory produced by depolarizing current injection. The role of potassium currents in the 5-HT effect was examined using whole cell voltage-clamp; in 6 of 9 neurons, 5-HT reversibly decreased inactivating potassium currents activated by depolarization. These experiments suggest that 5-HT's effect on the ramp trajectory may be mediated by a reduction of potassium currents activated by sub-threshold depolarization.",

keywords = "Modulation, Neostriatum, Potassium current, Serotonin, Voltage-clamp",

author = "Alessandro Stefani and {James Surmeier}, D. and Kitai, {S. T.}",

note = "Funding Information: This work was supported by USPHS Grants NS 20702 to S.T.K. and NS 26473 to S.T.K. and D.J.S. We wish to thank Drs. R. Foehring and C. Wilson for their critical reading of the manuscript.",

year = "1990",

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doi = "10.1016/0006-8993(90)90851-2",

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AU - Stefani, Alessandro

AU - James Surmeier, D.

AU - Kitai, S. T.

N1 - Funding Information: This work was supported by USPHS Grants NS 20702 to S.T.K. and NS 26473 to S.T.K. and D.J.S. We wish to thank Drs. R. Foehring and C. Wilson for their critical reading of the manuscript.

PY - 1990/10/8

Y1 - 1990/10/8

N2 - The physiological effects of serotonin (5-HT) on rat neostriatal neurons were investigated using current-clamp techniques in neostriatal slices and voltage-clamp techniques in acutely dissociated adult neostriatal neurons. In most neurons (35/51), bath-applied 5-HT (10-60 gmM) decreased the first spike latency and increased the evoked firing frequency. Membrane input resistance was also increased in most neurons (35/35) but could not explain the enhanced responsiveness. Tetrodotoxin, at concentrations sufficient to block spike production, did not block the ability of 5-HT to enhance the slow ramp-like voltage trajectory produced by depolarizing current injection. The role of potassium currents in the 5-HT effect was examined using whole cell voltage-clamp; in 6 of 9 neurons, 5-HT reversibly decreased inactivating potassium currents activated by depolarization. These experiments suggest that 5-HT's effect on the ramp trajectory may be mediated by a reduction of potassium currents activated by sub-threshold depolarization.

AB - The physiological effects of serotonin (5-HT) on rat neostriatal neurons were investigated using current-clamp techniques in neostriatal slices and voltage-clamp techniques in acutely dissociated adult neostriatal neurons. In most neurons (35/51), bath-applied 5-HT (10-60 gmM) decreased the first spike latency and increased the evoked firing frequency. Membrane input resistance was also increased in most neurons (35/35) but could not explain the enhanced responsiveness. Tetrodotoxin, at concentrations sufficient to block spike production, did not block the ability of 5-HT to enhance the slow ramp-like voltage trajectory produced by depolarizing current injection. The role of potassium currents in the 5-HT effect was examined using whole cell voltage-clamp; in 6 of 9 neurons, 5-HT reversibly decreased inactivating potassium currents activated by depolarization. These experiments suggest that 5-HT's effect on the ramp trajectory may be mediated by a reduction of potassium currents activated by sub-threshold depolarization.

KW - Modulation

KW - Neostriatum

KW - Potassium current

KW - Serotonin

KW - Voltage-clamp

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Serotonin enhances excitability in neostriatal neurons by reducing voltage-dependent potassium currents

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Keywords

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