Serotonin uptake and imipramine binding in blood platelets and brain of Fawn-hooded and Sprague Dawley rats

Ramesh C. Arora*, Ching Tong, Herbert L. Jackman, David Stoff, Herbert Y. Meltzer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

The kinetic parameters of serotonin (5-HT) uptake and imipramine binding of the synaptosomes and blood platelets of male Fawn-hooded rats, which have a 5-HT storage abnormality, and normal Sprague-Dawley rats were compared. The Vmax for 5-HT uptake of synaptosomes from Fawn-hooded rats was significantly greater than that of Sprague-Dawley rats whereas that of the platelets did not differ. The Km values of both the synaptosomes and platelets of the two rat strains were not significantly different. The Bmax values for 3H-imipramine binding on both platelets and brain of Fawn-hooded rats were significantly lower than those of the Sprague-Dawley rats. The Kd of imipramine binding to platelet membranes from Fawn-hooded rats was significantly less than that of the Sprague-Dawley rats but the Kd of the synaptosomes was not significantly different. These results indicate that 5-HT uptake and imipramine binding sites may be independently regulated and that platelets do not always manifest abnormalities present in brain 5-HT neurons and vice versa.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalLife Sciences
Volume33
Issue number5
DOIs
StatePublished - Aug 1 1983

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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