Serotonin uptake and imipramine binding in blood platelets and brain of Fawn-hooded and Sprague Dawley rats

Ramesh C. Arora; Ching Tong; Herbert L. Jackman; David Stoff; Herbert Y. Meltzer

doi:10.1016/0024-3205(83)90792-0

Serotonin uptake and imipramine binding in blood platelets and brain of Fawn-hooded and Sprague Dawley rats

Ramesh C. Arora^*, Ching Tong, Herbert L. Jackman, David Stoff, Herbert Y. Meltzer

^*Corresponding author for this work

Psychiatry and Behavioral Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

The kinetic parameters of serotonin (5-HT) uptake and imipramine binding of the synaptosomes and blood platelets of male Fawn-hooded rats, which have a 5-HT storage abnormality, and normal Sprague-Dawley rats were compared. The V_max for 5-HT uptake of synaptosomes from Fawn-hooded rats was significantly greater than that of Sprague-Dawley rats whereas that of the platelets did not differ. The K_m values of both the synaptosomes and platelets of the two rat strains were not significantly different. The B_max values for ³H-imipramine binding on both platelets and brain of Fawn-hooded rats were significantly lower than those of the Sprague-Dawley rats. The K_d of imipramine binding to platelet membranes from Fawn-hooded rats was significantly less than that of the Sprague-Dawley rats but the K_d of the synaptosomes was not significantly different. These results indicate that 5-HT uptake and imipramine binding sites may be independently regulated and that platelets do not always manifest abnormalities present in brain 5-HT neurons and vice versa.

Original language	English (US)
Pages (from-to)	437-442
Number of pages	6
Journal	Life Sciences
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/0024-3205(83)90792-0
State	Published - Aug 1 1983

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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title = "Serotonin uptake and imipramine binding in blood platelets and brain of Fawn-hooded and Sprague Dawley rats",

abstract = "The kinetic parameters of serotonin (5-HT) uptake and imipramine binding of the synaptosomes and blood platelets of male Fawn-hooded rats, which have a 5-HT storage abnormality, and normal Sprague-Dawley rats were compared. The Vmax for 5-HT uptake of synaptosomes from Fawn-hooded rats was significantly greater than that of Sprague-Dawley rats whereas that of the platelets did not differ. The Km values of both the synaptosomes and platelets of the two rat strains were not significantly different. The Bmax values for 3H-imipramine binding on both platelets and brain of Fawn-hooded rats were significantly lower than those of the Sprague-Dawley rats. The Kd of imipramine binding to platelet membranes from Fawn-hooded rats was significantly less than that of the Sprague-Dawley rats but the Kd of the synaptosomes was not significantly different. These results indicate that 5-HT uptake and imipramine binding sites may be independently regulated and that platelets do not always manifest abnormalities present in brain 5-HT neurons and vice versa.",

author = "Arora, {Ramesh C.} and Ching Tong and Jackman, {Herbert L.} and David Stoff and Meltzer, {Herbert Y.}",

note = "Funding Information: Supported, in part, by USPHS MH29,206 and MH30,938 and by the Department of Mental Health, State of Illinois. HYM is recipient of USPHS Research Scientist Award MH47,808.",

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AU - Stoff, David

AU - Meltzer, Herbert Y.

N1 - Funding Information: Supported, in part, by USPHS MH29,206 and MH30,938 and by the Department of Mental Health, State of Illinois. HYM is recipient of USPHS Research Scientist Award MH47,808.

PY - 1983/8/1

Y1 - 1983/8/1

N2 - The kinetic parameters of serotonin (5-HT) uptake and imipramine binding of the synaptosomes and blood platelets of male Fawn-hooded rats, which have a 5-HT storage abnormality, and normal Sprague-Dawley rats were compared. The Vmax for 5-HT uptake of synaptosomes from Fawn-hooded rats was significantly greater than that of Sprague-Dawley rats whereas that of the platelets did not differ. The Km values of both the synaptosomes and platelets of the two rat strains were not significantly different. The Bmax values for 3H-imipramine binding on both platelets and brain of Fawn-hooded rats were significantly lower than those of the Sprague-Dawley rats. The Kd of imipramine binding to platelet membranes from Fawn-hooded rats was significantly less than that of the Sprague-Dawley rats but the Kd of the synaptosomes was not significantly different. These results indicate that 5-HT uptake and imipramine binding sites may be independently regulated and that platelets do not always manifest abnormalities present in brain 5-HT neurons and vice versa.

AB - The kinetic parameters of serotonin (5-HT) uptake and imipramine binding of the synaptosomes and blood platelets of male Fawn-hooded rats, which have a 5-HT storage abnormality, and normal Sprague-Dawley rats were compared. The Vmax for 5-HT uptake of synaptosomes from Fawn-hooded rats was significantly greater than that of Sprague-Dawley rats whereas that of the platelets did not differ. The Km values of both the synaptosomes and platelets of the two rat strains were not significantly different. The Bmax values for 3H-imipramine binding on both platelets and brain of Fawn-hooded rats were significantly lower than those of the Sprague-Dawley rats. The Kd of imipramine binding to platelet membranes from Fawn-hooded rats was significantly less than that of the Sprague-Dawley rats but the Kd of the synaptosomes was not significantly different. These results indicate that 5-HT uptake and imipramine binding sites may be independently regulated and that platelets do not always manifest abnormalities present in brain 5-HT neurons and vice versa.

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Serotonin uptake and imipramine binding in blood platelets and brain of Fawn-hooded and Sprague Dawley rats

Abstract

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