Serum‐free media for cultures of primitive and mature hematopoietic cells

Craig E. Sandstrom, William M Miller, Eleftherios T. Papoutsakis*

*Corresponding author for this work

Research output: Contribution to journalReview article

28 Citations (Scopus)

Abstract

The in vitro culture of human hematopoietic cells has many research and therapeutic applications. Traditionally, human hematopoietic cultures have been conducted using serum‐containing media. The disadvantages inherent in the use of serum could be eliminated by the use of serum‐free media. In this review, we summarize and discuss the current status of serum‐free media for both mature and immature human hematopoietic cells. The mature hematopoietic cells discussed are of lymphoid (e.g., lymphokine activated killer cells and tumor infiltrating lymphocytes) and myeloid origin (e.g., monocytes/macrophages). The cultures of immature hematopoietic cells discussed are clonogenic and long‐term cultures. In addition, we briefly review the types of human hematopoietic cells, their clinical applications, and the basic strategies and components used to formulate serum‐free media, Finally, we outline future requirements and directions in the development of serum‐free media for primitive hematopoietic cells.

Original languageEnglish (US)
Pages (from-to)706-733
Number of pages28
JournalBiotechnology and Bioengineering
Volume43
Issue number8
DOIs
StatePublished - Jan 1 1994

Fingerprint

Culture Media
Lymphocytes
Lymphokines
Macrophages
Cell culture
Tumors
Therapeutic Human Experimentation
Tumor-Infiltrating Lymphocytes
Lymphokine-Activated Killer Cells
Monocytes
Serum
Direction compound

Keywords

  • cell culture
  • hematopoietic cells
  • serum‐free media

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Sandstrom, Craig E. ; Miller, William M ; Papoutsakis, Eleftherios T. / Serum‐free media for cultures of primitive and mature hematopoietic cells. In: Biotechnology and Bioengineering. 1994 ; Vol. 43, No. 8. pp. 706-733.
@article{6c0e54ca292244e5b8141651dcfe061d,
title = "Serum‐free media for cultures of primitive and mature hematopoietic cells",
abstract = "The in vitro culture of human hematopoietic cells has many research and therapeutic applications. Traditionally, human hematopoietic cultures have been conducted using serum‐containing media. The disadvantages inherent in the use of serum could be eliminated by the use of serum‐free media. In this review, we summarize and discuss the current status of serum‐free media for both mature and immature human hematopoietic cells. The mature hematopoietic cells discussed are of lymphoid (e.g., lymphokine activated killer cells and tumor infiltrating lymphocytes) and myeloid origin (e.g., monocytes/macrophages). The cultures of immature hematopoietic cells discussed are clonogenic and long‐term cultures. In addition, we briefly review the types of human hematopoietic cells, their clinical applications, and the basic strategies and components used to formulate serum‐free media, Finally, we outline future requirements and directions in the development of serum‐free media for primitive hematopoietic cells.",
keywords = "cell culture, hematopoietic cells, serum‐free media",
author = "Sandstrom, {Craig E.} and Miller, {William M} and Papoutsakis, {Eleftherios T.}",
year = "1994",
month = "1",
day = "1",
doi = "10.1002/bit.260430806",
language = "English (US)",
volume = "43",
pages = "706--733",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "8",

}

Serum‐free media for cultures of primitive and mature hematopoietic cells. / Sandstrom, Craig E.; Miller, William M; Papoutsakis, Eleftherios T.

In: Biotechnology and Bioengineering, Vol. 43, No. 8, 01.01.1994, p. 706-733.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Serum‐free media for cultures of primitive and mature hematopoietic cells

AU - Sandstrom, Craig E.

AU - Miller, William M

AU - Papoutsakis, Eleftherios T.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The in vitro culture of human hematopoietic cells has many research and therapeutic applications. Traditionally, human hematopoietic cultures have been conducted using serum‐containing media. The disadvantages inherent in the use of serum could be eliminated by the use of serum‐free media. In this review, we summarize and discuss the current status of serum‐free media for both mature and immature human hematopoietic cells. The mature hematopoietic cells discussed are of lymphoid (e.g., lymphokine activated killer cells and tumor infiltrating lymphocytes) and myeloid origin (e.g., monocytes/macrophages). The cultures of immature hematopoietic cells discussed are clonogenic and long‐term cultures. In addition, we briefly review the types of human hematopoietic cells, their clinical applications, and the basic strategies and components used to formulate serum‐free media, Finally, we outline future requirements and directions in the development of serum‐free media for primitive hematopoietic cells.

AB - The in vitro culture of human hematopoietic cells has many research and therapeutic applications. Traditionally, human hematopoietic cultures have been conducted using serum‐containing media. The disadvantages inherent in the use of serum could be eliminated by the use of serum‐free media. In this review, we summarize and discuss the current status of serum‐free media for both mature and immature human hematopoietic cells. The mature hematopoietic cells discussed are of lymphoid (e.g., lymphokine activated killer cells and tumor infiltrating lymphocytes) and myeloid origin (e.g., monocytes/macrophages). The cultures of immature hematopoietic cells discussed are clonogenic and long‐term cultures. In addition, we briefly review the types of human hematopoietic cells, their clinical applications, and the basic strategies and components used to formulate serum‐free media, Finally, we outline future requirements and directions in the development of serum‐free media for primitive hematopoietic cells.

KW - cell culture

KW - hematopoietic cells

KW - serum‐free media

UR - http://www.scopus.com/inward/record.url?scp=0028055759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028055759&partnerID=8YFLogxK

U2 - 10.1002/bit.260430806

DO - 10.1002/bit.260430806

M3 - Review article

VL - 43

SP - 706

EP - 733

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 8

ER -