Shark centra microanatomy and mineral density variation studied with laboratory microComputed Tomography

Paul E. Morse; Michala K. Stock; Kelsey C. James; Lisa J. Natanson; Stuart R. Stock

doi:10.1016/j.jsb.2022.107831

Shark centra microanatomy and mineral density variation studied with laboratory microComputed Tomography

Paul E. Morse, Michala K. Stock, Kelsey C. James, Lisa J. Natanson, Stuart R. Stock^*

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Centra of shark vertebrae from three species of Lamniformes (Alopias vulpinus, Carcharodon carcharias and Isurus oxyrinchus) and three species of Carcharhiniformes (Carcharhinus plumbeus, Carcharhinus obscurus and Prionace glauca) were imaged with laboratory microcomputed Tomography (microCT) using volume element (voxel) sizes between 16 and 24 µm. Linear attenuation coefficients were the same in the corpus calcarea (hour-glass-shaped cone) and intermedialia of the lamniforms but were smaller in the intermedialia than in the corpus calcarea of the carcharhiniforms. All centra contained growth bands which were visible as small changes in linear attenuation coefficient. In all six cases, the cross-sections of the cones were close to circular, and the cone angles matched those reported in the literature. Cartilage canals were a prominent structure in the intermedialia of all species, 3D renderings of centra of C. obscurus and I. oxyrinchus diameters showed these canals ran radially outward from the cone walls, and canal diameters were consistent with the limited numerical values in the literature. Somewhat higher calcification levels around the periphery of cartilage canals and of outer surfaces of the intermedialia and corpus calcerea suggest microstructural variation exists at scale below that which can be resolved in the present data sets.

Original language	English (US)
Article number	107831
Journal	Journal of Structural Biology
Volume	214
Issue number	1
DOIs	https://doi.org/10.1016/j.jsb.2022.107831
State	Published - Mar 2022

Funding

The authors thank Graham Davis (Queen Mary – University of London) and Ge Wang (Rensselaer Polytechnic Institute) for helpful discussion of statistics in computed tomography reconstructions. The microCT data were collected at the Duke University Shared Materials Instrumentation Facility (SMIF), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN), which is supported by the National Science Foundation (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). The authors thank Graham Davis (Queen Mary ? University of London) and Ge Wang (Rensselaer Polytechnic Institute) for helpful discussion of statistics in computed tomography reconstructions. The microCT data were collected at the Duke University Shared Materials Instrumentation Facility (SMIF), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN), which is supported by the National Science Foundation (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI).

Keywords

Carcharhiniformes
Centra
Lamniformes
Shark
Vertebra
microComputed Tomography

ASJC Scopus subject areas

Structural Biology

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10.1016/j.jsb.2022.107831

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@article{c5bcb77655c9425681813b6972f32c76,

title = "Shark centra microanatomy and mineral density variation studied with laboratory microComputed Tomography",

abstract = "Centra of shark vertebrae from three species of Lamniformes (Alopias vulpinus, Carcharodon carcharias and Isurus oxyrinchus) and three species of Carcharhiniformes (Carcharhinus plumbeus, Carcharhinus obscurus and Prionace glauca) were imaged with laboratory microcomputed Tomography (microCT) using volume element (voxel) sizes between 16 and 24 µm. Linear attenuation coefficients were the same in the corpus calcarea (hour-glass-shaped cone) and intermedialia of the lamniforms but were smaller in the intermedialia than in the corpus calcarea of the carcharhiniforms. All centra contained growth bands which were visible as small changes in linear attenuation coefficient. In all six cases, the cross-sections of the cones were close to circular, and the cone angles matched those reported in the literature. Cartilage canals were a prominent structure in the intermedialia of all species, 3D renderings of centra of C. obscurus and I. oxyrinchus diameters showed these canals ran radially outward from the cone walls, and canal diameters were consistent with the limited numerical values in the literature. Somewhat higher calcification levels around the periphery of cartilage canals and of outer surfaces of the intermedialia and corpus calcerea suggest microstructural variation exists at scale below that which can be resolved in the present data sets.",

keywords = "Carcharhiniformes, Centra, Lamniformes, Shark, Vertebra, microComputed Tomography",

author = "Morse, {Paul E.} and Stock, {Michala K.} and James, {Kelsey C.} and Natanson, {Lisa J.} and Stock, {Stuart R.}",

note = "Funding Information: The authors thank Graham Davis (Queen Mary – University of London) and Ge Wang (Rensselaer Polytechnic Institute) for helpful discussion of statistics in computed tomography reconstructions. The microCT data were collected at the Duke University Shared Materials Instrumentation Facility (SMIF), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN), which is supported by the National Science Foundation (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). Funding Information: The authors thank Graham Davis (Queen Mary ? University of London) and Ge Wang (Rensselaer Polytechnic Institute) for helpful discussion of statistics in computed tomography reconstructions. The microCT data were collected at the Duke University Shared Materials Instrumentation Facility (SMIF), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN), which is supported by the National Science Foundation (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",

year = "2022",

month = mar,

doi = "10.1016/j.jsb.2022.107831",

language = "English (US)",

volume = "214",

journal = "Journal of Structural Biology",

issn = "1047-8477",

publisher = "Academic Press Inc.",

number = "1",

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T1 - Shark centra microanatomy and mineral density variation studied with laboratory microComputed Tomography

AU - Morse, Paul E.

AU - Stock, Michala K.

AU - James, Kelsey C.

AU - Natanson, Lisa J.

AU - Stock, Stuart R.

N1 - Funding Information: The authors thank Graham Davis (Queen Mary – University of London) and Ge Wang (Rensselaer Polytechnic Institute) for helpful discussion of statistics in computed tomography reconstructions. The microCT data were collected at the Duke University Shared Materials Instrumentation Facility (SMIF), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN), which is supported by the National Science Foundation (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). Funding Information: The authors thank Graham Davis (Queen Mary ? University of London) and Ge Wang (Rensselaer Polytechnic Institute) for helpful discussion of statistics in computed tomography reconstructions. The microCT data were collected at the Duke University Shared Materials Instrumentation Facility (SMIF), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN), which is supported by the National Science Foundation (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). Publisher Copyright: © 2022 Elsevier Inc.

PY - 2022/3

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N2 - Centra of shark vertebrae from three species of Lamniformes (Alopias vulpinus, Carcharodon carcharias and Isurus oxyrinchus) and three species of Carcharhiniformes (Carcharhinus plumbeus, Carcharhinus obscurus and Prionace glauca) were imaged with laboratory microcomputed Tomography (microCT) using volume element (voxel) sizes between 16 and 24 µm. Linear attenuation coefficients were the same in the corpus calcarea (hour-glass-shaped cone) and intermedialia of the lamniforms but were smaller in the intermedialia than in the corpus calcarea of the carcharhiniforms. All centra contained growth bands which were visible as small changes in linear attenuation coefficient. In all six cases, the cross-sections of the cones were close to circular, and the cone angles matched those reported in the literature. Cartilage canals were a prominent structure in the intermedialia of all species, 3D renderings of centra of C. obscurus and I. oxyrinchus diameters showed these canals ran radially outward from the cone walls, and canal diameters were consistent with the limited numerical values in the literature. Somewhat higher calcification levels around the periphery of cartilage canals and of outer surfaces of the intermedialia and corpus calcerea suggest microstructural variation exists at scale below that which can be resolved in the present data sets.

AB - Centra of shark vertebrae from three species of Lamniformes (Alopias vulpinus, Carcharodon carcharias and Isurus oxyrinchus) and three species of Carcharhiniformes (Carcharhinus plumbeus, Carcharhinus obscurus and Prionace glauca) were imaged with laboratory microcomputed Tomography (microCT) using volume element (voxel) sizes between 16 and 24 µm. Linear attenuation coefficients were the same in the corpus calcarea (hour-glass-shaped cone) and intermedialia of the lamniforms but were smaller in the intermedialia than in the corpus calcarea of the carcharhiniforms. All centra contained growth bands which were visible as small changes in linear attenuation coefficient. In all six cases, the cross-sections of the cones were close to circular, and the cone angles matched those reported in the literature. Cartilage canals were a prominent structure in the intermedialia of all species, 3D renderings of centra of C. obscurus and I. oxyrinchus diameters showed these canals ran radially outward from the cone walls, and canal diameters were consistent with the limited numerical values in the literature. Somewhat higher calcification levels around the periphery of cartilage canals and of outer surfaces of the intermedialia and corpus calcerea suggest microstructural variation exists at scale below that which can be resolved in the present data sets.

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KW - Vertebra

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ER -

Shark centra microanatomy and mineral density variation studied with laboratory microComputed Tomography

Abstract

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Keywords

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