Short-term effects of amelogenin gene splice products A+4 and A-4 implanted in the exposed rat molar pulp

Nadège Jegat, Dominique Septier, Arthur Veis, Anne Poliard, Michel Goldberg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


In order to study the short-time effects of two bioactive low-molecular amelogenins A+4 and A-4, half-moon cavities were prepared in the mesial aspect of the first maxillary molars, and after pulp exposure, agarose beads alone (controls) or beads soaked in A+4 or A-4 (experimental) were implanted into the pulp. After 1, 3 or 7 days, the rats were killed and the teeth studied by immunohistochemistry. Cell proliferation was studied by PCNA labeling, positive at 3 days, but decreasing at day 7 for A+4, whilst constantly high between 3 and 7 days for A-4. The differentiation toward the osteo/odontoblast lineage shown by RP59 labeling was more apparent for A-4 compared with A+4. Osteopontin-positive cells were alike at days 3 and 7 for A-4. In contrast, for A+4, the weak labeling detected at day 3 became stronger at day 7. Dentin sialoprotein (DSP), an in vivo odontoblast marker, was not detectable until day 7 where a few cells became DSP positive after A-4 stimulation, but not for A+4. These results suggest that A +/- 4 promote the proliferation of some pulp cells. Some of them further differentiate into osteoblast-like progenitors, the effects being more precocious for A-4 (day 3) compared with A+4 (day 7). The present data suggest that A +/- 4 promote early recruitment of osteogenic progenitors, and evidence functional differences between A+4 and A-4.

Original languageEnglish (US)
Article number40
JournalHead and Face Medicine
Issue number1
StatePublished - 2007

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Short-term effects of amelogenin gene splice products A+4 and A-4 implanted in the exposed rat molar pulp'. Together they form a unique fingerprint.

Cite this