Abstract
Shuttle mutagenesis is a method for producing stable mini-transposon (mTn) insertions into the genome of Neisseria gonorrhoeae (gonococcus, Gc) and other microbes. Using an mTn3 derivative, we have produced an mTn (mTnCmPhoA) which contains a phoA' gene lacking its N-terminal signal sequence useful for isolating genes which encode exported proteins, mTnCmPhoA was characterized in Gc and Escherichia coli using a cloned target containing the Gc genes, opaE1, pilA and piIB. PhoA+ Gc containing pi1B::mTnCmPhoA insertions confirm that Pi1B is an exported protein in Gc. This system will be useful for isolating and characterizing extracytoplasmic virulence factors from Gc and other bacterial pathogens.
Original language | English (US) |
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Pages (from-to) | 101-106 |
Number of pages | 6 |
Journal | Gene |
Volume | 155 |
Issue number | 1 |
DOIs | |
State | Published - Mar 21 1995 |
Keywords
- Exported protein
- opaE1
- pathogen
- pilA
- pilB
- virulence factor
ASJC Scopus subject areas
- Genetics