Signaling Networks among Stem Cell Precursors, Transit-Amplifying Progenitors, and their Niche in Developing Hair Follicles

Amélie Rezza, Zichen Wang, Rachel Sennett, Wenlian Qiao, Dongmei Wang, Nicholas Heitman, Ka Wai Mok, Carlos Clavel, Rui Yi, Peter Zandstra, Avi Ma'ayan, Michael Rendl*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

The hair follicle (HF) is a complex miniorgan that serves as an ideal model system to study stem cell (SC) interactions with the niche during growth and regeneration. Dermal papilla (DP) cells are required for SC activation during the adult hair cycle, but signal exchange between niche and SC precursors/transit-amplifying cell (TAC) progenitors that regulates HF morphogenetic growth is largely unknown. Here we use six transgenic reporters to isolate 14 major skin and HF cell populations. With next-generation RNA sequencing, we characterize their transcriptomes and define unique molecular signatures. SC precursors, TACs, and the DP niche express a plethora of ligands and receptors. Signaling interaction network analysis reveals a bird's-eye view of pathways implicated in epithelial-mesenchymal interactions. Using a systematic tissue-wide approach, this work provides a comprehensive platform, linked to an interactive online database, to identify and further explore the SC/TAC/niche crosstalk regulating HF growth.

Original languageEnglish (US)
Pages (from-to)3001-3018
Number of pages18
JournalCell reports
Volume14
Issue number12
DOIs
StatePublished - Mar 29 2016
Externally publishedYes

Keywords

  • Big data
  • Dermal papilla
  • Epithelial-mesenchymal interactions
  • Hair follicle
  • Hair growth
  • Progenitors
  • Signaling networks
  • Stem cell niche
  • Stem cells
  • Systems biology
  • Transcriptomics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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