TY - JOUR
T1 - Simian virus 40 large-T bypasses the translational block imposed by the phosphorylation of elF-2α
AU - Swaminathan, Sathyamangalam
AU - Rajan, Prithi
AU - Savinova, Olga
AU - Jagus, Rosemary
AU - Thimmapaya, Bayar
N1 - Funding Information:
This work was supported by NIH Grants AI18029 and 20156 (B.T.) and NSF MCB 9317264 (R.J.). We thank Dr. Lynn O’Brien from the laboratory of the late Dr. Henshaw for providing monoclonal antibody to eIF-2α.
PY - 1996/5/1
Y1 - 1996/5/1
N2 - One of the cellular defense mechanisms against virus infection is mediated by activating the interferon-induced, double-stranded-RNA-activated protein kinase, PKR. Upon activation, PKR phosphorylates and thereby inactivates the protein synthesis initiation factor, elF-2, leading to cessation of protein synthesis. Viruses have evolved diverse strategies to counteract this cellular antiviral response. A majority of these strategies target PKR to prevent its activation. Recently, we showed that simian virus40 (SV40) large-T antigen reverses PKR-mediated translational inhibition at a step downstream of PKR activation (Rajan et al., J. Virol. 69, 785-795, 1995). In this paper, we present evidence showing that SV40 can restore efficient translation in cells despite the elevated levels of phosphorylated elF-2α resulting from PKR activation. Thus, SV40 large-T-mediated translational rescue occurs at a step downstream of elF-2α phosphorylation.
AB - One of the cellular defense mechanisms against virus infection is mediated by activating the interferon-induced, double-stranded-RNA-activated protein kinase, PKR. Upon activation, PKR phosphorylates and thereby inactivates the protein synthesis initiation factor, elF-2, leading to cessation of protein synthesis. Viruses have evolved diverse strategies to counteract this cellular antiviral response. A majority of these strategies target PKR to prevent its activation. Recently, we showed that simian virus40 (SV40) large-T antigen reverses PKR-mediated translational inhibition at a step downstream of PKR activation (Rajan et al., J. Virol. 69, 785-795, 1995). In this paper, we present evidence showing that SV40 can restore efficient translation in cells despite the elevated levels of phosphorylated elF-2α resulting from PKR activation. Thus, SV40 large-T-mediated translational rescue occurs at a step downstream of elF-2α phosphorylation.
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U2 - 10.1006/viro.1996.0255
DO - 10.1006/viro.1996.0255
M3 - Article
C2 - 8623549
AN - SCOPUS:0029996794
SN - 0042-6822
VL - 219
SP - 321
EP - 323
JO - Virology
JF - Virology
IS - 1
ER -